We narrowed to 18,451 results for: Met

1. pET21-pelB-LaG-2

   Plasmid

   #172739

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-2
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Clover-ZO1-C-14

   Plasmid

   #56375

   Purpose

   Localization: Tight Junctions, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   ZO1 (TJP1 Human)
   

   Tags

   Clover

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 18, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

   Plasmid

   #69226

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS3 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFJ914

   Plasmid

   #44489

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
   

   Use

   Gateway expression clone

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNB655

   Plasmid

   #60142

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Expression

   Yeast

   Mutation

   A4V

   Promoter

   MET17pr

   Available Since

   Feb. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET-spa-GCaMP6f

   Plasmid

   #67563

   Purpose

   bacterial expression of a photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   spa-GCaMP6f
   

   Tags

   HIS-tag

   Expression

   Bacterial

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBabe-SIRT3

   Plasmid

   #66167

   Purpose

   Expresses SIRT3 via retroviral method

   Depositor

   Ronald Kahn

   Article

   Jing et al Diabetes. 2013 Oct;62(10):3404-17. doi:

   Insert

   SIRT3 (Sirt3 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   L235P

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

   Plasmid

   #69224

   Purpose

   Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS and Zif268

   Expression

   Mammalian

   Mutation

   Arginine 1335 is changed to Lysine

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. fgfprom luc

   Plasmid

   #69446

   Purpose

   basal plasmid containing minimal fgf4 promoter used for testing enhancer activity of DNAs inserted immediately upstream of the promoter.

   Depositor

   Lisa Dailey

   Article

   Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

   Insert

   minimal fgf4 promoter
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mIFP-MannII-N-10

    Plasmid

    #56229

    Purpose

    Localization: Mannosidase/Golgi, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    MannII
    

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pBEST-HipO

    Plasmid

    #114599

    Purpose

    Strong constitutive expression of metabolic enzyme, HipO, for cell-free expression

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    HipO
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Promoter

    OR2-OR1-Pr

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSP1D1T1

    Plasmid

    #97327

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1T1
    

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of T residue after T25

    Promoter

    T7

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. TALEN-CCR5L18-CtermCan

    Plasmid

    #51438

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with canonical C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) Can C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. TALEN-CCR5R18-CtermCan

    Plasmid

    #51439

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with canonical C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) Can C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS

    Plasmid

    #69223

    Purpose

    Expresses R1335K mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNB695

    Plasmid

    #60144

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Promoter

    MET17pr

    Available Since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNB697

    Plasmid

    #60146

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    RdLuc
    

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Promoter

    MET17pr

    Available Since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. Clover-SiT-N-15

    Plasmid

    #56306

    Purpose

    Localization: Golgi TGN, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    SiT (ST6GAL1 Human)
    

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    aa 1-45 of SiT

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. AB.pCCL.sin.cPPT.U6.miR-24-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46602

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-24 Decoy (Mir24-1 Mouse, Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

20. AB.pCCL.sin.cPPT.GFP.miR-370-5p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85854

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-370-5p Sensor (MIR370 Human)
    

    Use

    Lentiviral

    Promoter

    PGK

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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