We narrowed to 10,382 results for: plasmids 101

1. pDMJ2-SARS-CoV-2-Spike (B.1-10b)

   Plasmid

   #241035

   Purpose

   Expresses SARS-CoV-2 spike (B.1-10b) protein for creating pseudotyped virus

   Depositor

   Jeremy Luban

   Article

   Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

   Insert

   Spike (B.1-10b) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K113T,K147N,S155R,Y248D,T478K,E484V,F490R,S494R,K…

   Promoter

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDMJ2-SARS-CoV-2-Spike (B.1-10d)

   Plasmid

   #241037

   Purpose

   Expresses SARS-CoV-2 spike (B.1-10d) protein for creating pseudotyped virus

   Depositor

   Jeremy Luban

   Article

   Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

   Insert

   Spike (B.1-10d) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H69G,Y248D,R403T,N439R,T478K,E484V,F486E,F490R,S4…

   Promoter

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDMJ2-SARS-CoV-2-Spike (B.1-10a)

   Plasmid

   #241034

   Purpose

   Expresses SARS-CoV-2 spike (B.1-10a) protein for creating pseudotyped virus

   Depositor

   Jeremy Luban

   Article

   Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

   Insert

   Spike (B.1-10a) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H69G,K113T,K147N,S155R,T478K,E484V,F490R,S494R,K5…

   Promoter

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTwist-SARS-CoV-2 Spike-deltaC18 (P.1) - No RBD Mutations

   Plasmid

   #212384

   Purpose

   Encodes SARS-CoV-2 spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V11…

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIG-748_HA-GD2-28z_CAR_BATF

   Plasmid

   #207488

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   BATF, HA-GD2-28z_CAR (BATF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIG-805_HA-GD2-28z_CAR_BATF-RFP

   Plasmid

   #207492

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   BATF-RFP, HA-GD2-28z_CAR (BATF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIG-755_HA-GD2-28z_CAR_RFP-JUN

   Plasmid

   #207494

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   RFP-JUN, HA-GD2-28z_CAR (JUN Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Spike Display_Part 1-5 DO

   Plasmid

   #172726

   Purpose

   Encodes a sfGFP dropout expression cassette in place of Parts 1-5 to be used in the Spike Display system

   Depositor

   IIya Finkelstein

   Article

   Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

   Insert

   SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

   Expression

   Mammalian

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   CMV

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. cmv-sfGFP-GB1-scAB

   Plasmid

   #185794

   Purpose

   scFv GFP compnent for SunRISER

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   sfGFP-GB1-scAB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAPInt-sfGFP-G418

    Plasmid

    #236481

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on G418.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pV1090

    Plasmid

    #111427

    Purpose

    gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAPInt-mNeonGreen-G418

    Plasmid

    #236484

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on G418.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAPInt-mStayGold-G418

    Plasmid

    #236487

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on G418.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAPInt-mCherry-G418

    Plasmid

    #236490

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on G418.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAPInt-Dendra2-G418

    Plasmid

    #236496

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on G418.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    Dendra2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAPInt-mScarlet-G418

    Plasmid

    #236493

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on G418.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAPInt-GFP-G418

    Plasmid

    #236478

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-empty

    Plasmid

    #239099

    Purpose

    pGL3 luciferase vector with no promoter.

    Depositor

    David Cobrinik

    Article

    Shayler et al eLife 13:RP101918

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAG-Electra1-P2A-EGFP

    Plasmid

    #184935

    Purpose

    Expresses blue fluorescent protein Electra1 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra1-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs-hOCT3/4

    Plasmid

    #17217

    Purpose

    Retroviral expression of human OCT3/4

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    OCT3/4 (POU5F1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge