We narrowed to 38,405 results for: ANT

1. pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

   Plasmid

   #139484

   Purpose

   PCR template for mEGFP gene knock-in donor vector

   Depositor

   Kazuhiro Aoki

   Article

   Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

   Insert

   mEGFP-loxP-P2A-dTKneo-pA-loxP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJAT31

   Plasmid

   #204291

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pB-CMPE-ePPEplus

   Plasmid

   #205246

   Purpose

   For plant prime editing in wheat plants

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Promoter

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDx_mScarlet3-SYFP2

   Plasmid

   #189764

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-SYFP2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK1289-AAV-dCjCas9

   Plasmid

   #223138

   Purpose

   AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCer-TG2-YFP

   Plasmid

   #85471

   Purpose

   TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

   Depositor

   Jeffrey Keillor, Ray Truant

   Article

   Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

   Insert

   hTG2 (TGM2 Human)
   

   Use

   Tags

   EYFP and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mH2A1.1-CT-GFP

   Plasmid

   #45167

   Depositor

   Hunt Willard, Brian Chadwick

   Article

   Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

   Insert

   H2Afy (MACROH2A1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

   Plasmid

   #223160

   Purpose

   Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   dGFP-P2A-NanoLuc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pet28a-His6-Keap1

   Plasmid

   #62454

   Purpose

   His6 tagged Keap1 for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGWB405

    Plasmid

    #74799

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGWB453

    Plasmid

    #74835

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCIpA102-G-HLA-A2_GFP

    Plasmid

    #85162

    Purpose

    for mRNA expression of HLA.A2-EGFP

    Depositor

    Tone Fredsvik Gregers, Sébastien Walchli

    Article

    Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

    Insert

    HLA.A2 (HLA-A Human)
    

    Use

    Mrna expression

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGWB553

    Plasmid

    #74883

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDGE334

    Plasmid

    #153243

    Purpose

    M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDGE337

    Plasmid

    #153246

    Purpose

    M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HBT-pcoCas9

    Plasmid

    #52254

    Purpose

    transient expression of pcoCas9 gene in plant cells

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    pcoCas9
    

    Use

    CRISPR; Plant expression

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    hybrid constitutive promoter 35SPPDK

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSG5-ERRbeta2

    Plasmid

    #52186

    Purpose

    Expresses human ERRbeta2 splice variant in mammalian cells

    Depositor

    Rebecca Riggins

    Article

    Heckler et al Cell Cycle. 2014 Dec 12:0.

    Insert

    Estrogen-related receptor beta (ESRRB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type sequence; codon optimized to exclude Ec…

    Promoter

    SV40

    Available since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSMART-sgRNA (Sp)

    Plasmid

    #80427

    Purpose

    U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-c-Met

    Plasmid

    #31786

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGWB535

    Plasmid

    #74873

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBITE-EGFRvIII-F263-OKT3

    Plasmid

    #190680

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. dpYPQ230-D156R-CBE4

    Plasmid

    #195369

    Purpose

    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    hA3A/Y130F-(GGGGS)x6-dLbCas12a-D156R-UGI
    

    Use

    CRISPR; Gateway compatible ha3a/y130f-(ggggs)x6-d…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    ZmUBI1

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pROF417

    Plasmid

    #155335

    Purpose

    Constitutive expression LFY-VP16-2A-RLuc in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. dpYPQ230-D156R-ABE5

    Plasmid

    #195371

    Purpose

    dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    ecTadA8e-(GGGGS)x8-dLbCas12a-D156R
    

    Use

    CRISPR; Gateway compatible ectada8e-(ggggs)x8-dlb…

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    ZmUBI1

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGWB504

    Plasmid

    #74846

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAcrVIB1

    Plasmid

    #184566

    Purpose

    Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    anti-CRISPR protein inhibiting Cas13b nucleases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPROEX-HTc-Flag3-Nrf2

    Plasmid

    #21553

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
    

    Use

    Tags

    6xHis and Flag3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

28. pGWB441

    Plasmid

    #74827

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDGE476

    Plasmid

    #153267

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-PKCBII

    Plasmid

    #195232

    Purpose

    Mammalian expression vector containing GFP tagged PKCBII.

    Depositor

    Keith Mostov, David Bryant

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PKCB2 (PRKCB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGWB442

    Plasmid

    #74828

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. E3020X

    Plasmid

    #21366

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed E3020 to stop codon

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

33. pXEE401B

    Plasmid

    #91716

    Purpose

    CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. A3A-PBE

    Plasmid

    #119768

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A and E945K in wild type …

    Promoter

    maize Ubiquitin-1

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJAT7

    Plasmid

    #204292

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGREAT2

    Plasmid

    #170890

    Purpose

    GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    p35SoTMV

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGREAT1

    Plasmid

    #170889

    Purpose

    GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pNOS

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. μ2-HA-D176A

    Plasmid

    #32754

    Depositor

    Alexander Sorkin

    Article

    Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

    Insert

    μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D176A

    Promoter

    CMV

    Available since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pGWB541

    Plasmid

    #74875

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPML1 (GB0045)

    Plasmid

    #68164

    Purpose

    Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtML1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge