We narrowed to 75,864 results for: Rest

1. FLX1.8CMVMitoTurboRFP

   Plasmid

   #28131

   Depositor

   Geoffrey Owens

   Article

   Chen et al PLoS One. 2008 . 3(7):e2804.

   Insert

   MitoTurboRFP
   

   Use

   Lentiviral

   Tags

   COX VIII signal sequence

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-centrin-1

   Plasmid

   #72641

   Purpose

   The plasmid encodes centrin-1 with an N-terminal EGFP tag

   Depositor

   Michel Bornens

   Insert

   centrin-1 (CETN1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hTLR2 flag

   Plasmid

   #13082

   Depositor

   Ruslan Medzhitov

   Insert

   TLR2 (TLR2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Amino acids 1-16 have been replaced with mammalia…

   Promoter

   Available since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40]

   Plasmid

   #86453

   Purpose

   UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker)

   Depositor

   Gregor Bucher

   Insert

   3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

   Plasmid

   #174893

   Purpose

   amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Insert

   mCherry and EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CX3CR1-EGFP

   Plasmid

   #24061

   Depositor

   Dan Littman

   Insert

   CX3CR1 (Cx3cr1 Mouse)
   

   Use

   Mouse Targeting

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLNCX chick src Y527F

   Plasmid

   #13660

   Depositor

   Joan Brugge

   Insert

   src Y527F (SRC Chicken)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Y527F. Constitutive active mutant.

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBABE puro cyclinD1 HA

   Plasmid

   #9050

   Depositor

   William Hahn

   Insert

   cyclin D1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYPet-His

   Plasmid

   #14031

   Depositor

   Patrick Daugherty

   Article

   Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

   Insert

   YPet
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. tet-pLKO.puro_shGFP

    Plasmid

    #110421

    Purpose

    shGFP control, silence GFP gene, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO (RNA PolIII)

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FLAG-Foxo4

    Plasmid

    #17549

    Depositor

    Domenico Accili

    Insert

    FoxO4 (FOXO4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLinker-6xHA-HXGPRT-LoxP

    Plasmid

    #86552

    Purpose

    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma

    Depositor

    David Sibley

    Insert

    Linker-6xHA-HGPRT-LoxP
    

    Use

    Expression in toxoplasma gondii

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZE21-GFPaav

    Plasmid

    #26643

    Depositor

    Michael Elowitz

    Article

    Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

    Insert

    pZE21-GFPaav
    

    Use

    Tags

    GFPaav

    Expression

    Bacterial

    Mutation

    na

    Promoter

    Available since

    June 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti6MFV SLC25A4

    Plasmid

    #194133

    Purpose

    Expresses ANT1 (SLC25A4) protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC25A4 (SLC25A4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG CMV N-HA Puro

    Plasmid

    #237879

    Purpose

    Empty mammalian expression plasmid for cloning N-terminally HA-tagged proteins

    Depositor

    Meghan Rego, Addgene Research Program

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGEX4T1-Opt WWP1

    Plasmid

    #179327

    Purpose

    E. coli optimized expression of GST-fusion of human WWP1

    Depositor

    Arthur Haas

    Insert

    WWP1 (WWP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli

    Promoter

    Available since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJA54 HLA-A*03:01

    Plasmid

    #180451

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*03:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(-) anti-Her2 (FRP5) scFv-Fc

    Plasmid

    #183249

    Purpose

    Soluble expression of anti-human Her2 scFvFc (clone FRP5). Human IgG1 Fc.

    Depositor

    Alexander McLellan

    Insert

    Anti-Her2 scFvFc (FRP5)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mTLR4 flag

    Plasmid

    #13087

    Depositor

    Ruslan Medzhitov

    Insert

    TLR4 (Tlr4 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino acids 1-19 have been replaced with mammalia…

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. nuc-mCherry-pTRIP

    Plasmid

    #163520

    Purpose

    Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells

    Depositor

    Thomas Weber

    Insert

    nucleus targeted mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. eSpCas9(1.1)-T2A-Puro

    Plasmid

    #101039

    Purpose

    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

    Depositor

    Andrea Németh

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFLAG and T2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1(-) FMC-63 scFv-Fc (huFc)

    Plasmid

    #183252

    Purpose

    Soluble expression of the murine anti-human CD19 scFv-Fc (clone FMC-63). Human IgG1 Fc.

    Depositor

    Alexander McLellan

    Insert

    anti-CD19 scFvFc (FMC-63)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-syn-DIO-axon-jGCaMP8f

    Plasmid

    #220531

    Purpose

    For Cre-dependent enriched expression of GCaMP8f in axons

    Depositor

    Lin Tian

    Insert

    Axon-DIO-GCaMP8f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET His6 MBP prescission LIC cloning vector (HMPKS)

    Plasmid

    #29721

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, MBP, and Prescission cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-2T-Rac1-wt

    Plasmid

    #12977

    Depositor

    Gary Bokoch

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

27. AbVec2.0-mIghg3

    Plasmid

    #127160

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg3 (Ighg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CMV-iRFP713(V256C)-P2A-EGFP

    Plasmid

    #198071

    Purpose

    The AAV vector for iRFP713(V256C) and EGFP

    Depositor

    Michiyuki Matsuda

    Insert

    iRFP713(V256C)
    

    Use

    AAV

    Tags

    P2A-EGFP

    Expression

    Mutation

    V256C mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFA6a-link-ymScarletI-SpHis5

    Plasmid

    #219844

    Purpose

    tagging vector to tag genes with ymScarletI and a HIS5 gene

    Depositor

    Bas Teusink

    Insert

    ymScarletI-SpHis5
    

    Use

    Tags

    ymScarletI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pcDNA3.1(-)-flag-UBR2

    Plasmid

    #50573

    Purpose

    Expresses FLAG-tagged mouse UBR2 in mammalian cells.

    Depositor

    Alexander Varshavsky

    Insert

    UBR2 (Ubr2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

31. pEGFP-C1-TULP3

    Plasmid

    #218741

    Purpose

    Expresses C-terminally EGFP-tagged TULP3 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    TULP3 (TULP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21a AHU

    Plasmid

    #26640

    Depositor

    Phoebe Rice

    Article

    Swinger et al EMBO J. 2003 Jul 15. 22(14):3749-60.

    Insert

    AHU
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGHE-PdNIP2-1

    Plasmid

    #108447

    Purpose

    PdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.

    Depositor

    Richard Bélanger

    Insert

    PdNIP2-1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pT7

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFA6a-link-ymTurquoise2-Kan

    Plasmid

    #224092

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with Kan selection

    Depositor

    Bas Teusink

    Insert

    ymTurquoise2
    

    Use

    Tags

    ymTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV6-XL4 FLAG-NGRN-Fc

    Plasmid

    #115773

    Purpose

    Expression of the extracellular domain of NGRN with N-terminal FLAG tag and C-terminal hIgG tag.

    Depositor

    Davide Comoletti

    Insert

    NGRN (NGRN Human)
    

    Use

    Tags

    FLAG and hIgG/Fc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTCF236 HLA-B*40:01

    Plasmid

    #180463

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*40:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET21b-Casp1-His

    Plasmid

    #11809

    Depositor

    Guy Salvesen

    Insert

    Caspase-1 (CASP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTCF7 HLA-B*07:02

    Plasmid

    #180446

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*07:02
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEGFP-U6

    Plasmid

    #19799

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pRZ

    Plasmid

    #27663

    Depositor

    Graeme Conn

    Article

    Walker et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Type

    Empty backbone

    Use

    Rna transcription

    Tags

    HDV Ribozyme

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. M38 TOP-dGFP

    Plasmid

    #17114

    Depositor

    Randall Moon

    Insert

    Tcf/Lef sites (Ctnnb1 )
    

    Use

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pOpen-T4gene30

    Plasmid

    #165554

    Purpose

    Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    T4-DNA Ligase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pBABE.is4-mOpn

    Plasmid

    #74410

    Purpose

    Expresses mouse Osteopontin from transcript tv4 in mammalian cells

    Depositor

    Georgios Stathopoulos

    Insert

    mouse Opn (Spp1 Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    MMLV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA3-BG4-NLS-mCer

    Plasmid

    #211448

    Purpose

    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

    Depositor

    Helmut Pospiech

    Insert

    Anti-DNA G-quadruplex single chain antibody BG4
    

    Use

    Tags

    NLS-mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCIG3 Rab5CA

    Plasmid

    #78268

    Purpose

    Rab5 Constitutively active (Q79L) expression

    Depositor

    Felicia Goodrum

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Q79L

    Promoter

    CMV, Lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. AAV-pgk-Cre

    Plasmid

    #24593

    Depositor

    Patrick Aebischer

    Has Service

    AAV Retrograde

    Insert

    Cre recombinase (codon optimized)
    

    Use

    AAV and Cre/Lox; Adeno-associated

    Tags

    Expression

    Mutation

    Promoter

    pgk

    Available since

    May 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3-ps-CFP

    Plasmid

    #13034

    Purpose

    Expresses photo-switchable CFP in mammalian cells. Can undergo irreversible photoconversion from cyan to green fluorescence

    Depositor

    Doug Golenbock

    Insert

    photo-switchable cyan fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAAV.TBG.PI.FLPo.rBG

    Plasmid

    #236288

    Purpose

    AAV vector expressing FLPo from TBG promoter

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    FLPo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TBG

    Available since

    May 20, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. OTUD5

    Plasmid

    #28270

    Depositor

    Cheryl Arrowsmith

    Insert

    OTUD5 (OTUD5 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    OTU DOMAIN (residues 172-339).

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

50. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid

    #191442

    Purpose

    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hROSA26 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge