We narrowed to 5,537 results for: mcs

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pCAB1-GW

Plasmid

#209371

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by CAB1 promoter

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pSKAP

Plasmid

#122702

Purpose

Designed for the incorporation of DNA barcode sequences for subsequent allelic exchange on bacterial chromosomes.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Type

Empty backbone

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV610

Plasmid

#119018

Purpose

RMCE vector containing inverted TP901-1 attB sites flanking large MCS

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attB sites

Use

Fruit fly rmce

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_Xho

Plasmid

#110223

Purpose

Cre-expression plasmid for cassette exchange but MCS modified compared to pAW8

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-323

Plasmid

#68789

Purpose

Sensor pair 2 V2

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 2 V2
N-Terminal Sensor 2 V2

Tags

NLS, flag, and nls

Expression

Mammalian

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-248

Plasmid

#68762

Purpose

ZF9-TF-V3 with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF9-TF-V3
ZF9-TF-V3
mCherry

Tags

NLS and flag

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-115

Plasmid

#68730

Purpose

ZF2-TF with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF2-VP64
mCherry

Tags

FLAG and NLS

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-117

Plasmid

#68732

Purpose

ZF4-TF with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF4-VP64
mCherry

Tags

FLAG and NLS

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-128

Plasmid

#68734

Purpose

ZF6-TF with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF6-VP64
mCherry

Tags

NLS and flag

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-130

Plasmid

#68735

Purpose

ZF7-TF with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF7-VP64
mCherry

Tags

NLS and flag

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-131

Plasmid

#68736

Purpose

ZF8-TF with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF8-VP64
mCherry

Tags

NLS and flag

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-132

Plasmid

#68729

Purpose

ZF1-TF with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF1-VP64
mCherry

Tags

FLAG and NLS

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pGP-pcDNA3.1 Puro-CAG-Voltron2

Plasmid

#172909

Purpose

Mammalian expression of voltage sensor

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Voltron2

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHIS-MDH1-HIS

Plasmid

#184558

Purpose

Bacterial expression of human MDH1 with HIS Tag

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Insert

Malate dehydrogenase 1 (MDH1 Human)

Tags

6xHIS

Expression

Bacterial

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

Plasmid

#179905

Purpose

Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

Insert

SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…

Promoter

CAG

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDNA5-FRT/TO-FLAG-TONSL

Plasmid

#221011

Purpose

mammalian expression vector of Flag tagged TONSL siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

TONSL (TONSL Human)

Tags

Flag

Expression

Mammalian

Mutation

silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

Promoter

CMV

Available Since

June 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pINTO-N3::hTET3 HxD

Plasmid

#136365

Purpose

Expresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

HxD (H1077Y, D1079A)

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJT230 lenti pEF-rTetR(SE-G72P)-3XFLAG-NCOA3-TAD-T2A-mCherry-BSD-WPRE

Plasmid

#188763

Purpose

Lentiviral backbone containing NCOA3 as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

NCOA3 (NCOA3 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

Plasmid

#156415

Purpose

kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12D (KRAS Human)

Use

Zebrafish transgenesis

Tags

mTagBFP2

Mutation

mutant KRAS4A-G12D

Promoter

kdrl

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)

Plasmid

#180468

Purpose

kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

KRAS4A (KRAS Human)

Use

Zebrafish transgenesis

Tags

V5-mScarlet-I

Promoter

kdrl

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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