We narrowed to 8,593 results for: Set

1. Rufy3 scFv [N483/84]

   Plasmid

   #190559

   Purpose

   Mammalian Expression of Rufy3 scFV. Derived from hybridoma N483/84.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Rufy3 (Mus musculus) recombinant scFV (Rufy3 Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. IL2-mCherry-rGBD in pmCherryN1

   Plasmid

   #187286

   Purpose

   Express IL2-Rhotekin GTPase-binding domain-mCherry

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   interleukin-2 receptor alpha-subunit, Rhotekin (IL2, Rtkn Mouse, Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Anti-SAPAP1/2 [N238/31R]

   Plasmid

   #182110

   Purpose

   Mammalian Expression Plasmid of anti-SAPAP1/2 (). Derived from hybridoma N238/31.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-SAPAP1/2 () recombinant mouse monoclonal antibody
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Parvalbumin scFv [L114/81]

   Plasmid

   #182073

   Purpose

   Mammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/81.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)
   

   Tags

   HA, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

   Plasmid

   #113696

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

   Plasmid

   #113695

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. N295A (EcN), E166Q (EcN) MetNI LmH

   Plasmid

   #118269

   Purpose

   N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetI (metI Escherichia coli)
   MetN (E166Q ; N295A) (metN Escherichia coli)
   

   Tags

   6x His Tag

   Expression

   Bacterial

   Mutation

   Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…

   Promoter

   T7 Promoter

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 Flag YFP hNKCC1 C820S (NT573)

   Plasmid

   #49075

   Purpose

   Expresses human NKCC1 C820S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   C820S in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Flag YFP hNKCC1 C1052S (NT674)

   Plasmid

   #49076

   Purpose

   Expresses human NKCC1 C1052S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   C1052S in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 Flag YFP hNKCC1 C791S (NT572)

    Plasmid

    #49074

    Purpose

    Expresses human NKCC1 C791S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C791S in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 Flag YFP hNKCC1 C543M (NT360)

    Plasmid

    #49068

    Purpose

    Expresses human NKCC1 C543M mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C543M in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 Flag YFP hNKCC1 C723S,C724V (NT501)

    Plasmid

    #49073

    Purpose

    Expresses human NKCC1 C723S and C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C723S,C724V in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 Flag YFP hNKCC1 C630S (NT400)

    Plasmid

    #49072

    Purpose

    Expresses human NKCC1 C630S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C630S in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 Flag YFP hNKCC1 C630A (NT399)

    Plasmid

    #49071

    Purpose

    Expresses human NKCC1 C630A mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C630A in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 Flag YFP hNKCC1 C295A (NT104)

    Plasmid

    #49067

    Purpose

    Expresses human NKCC1 C295A mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C295A in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 Flag YFP hNKCC1 C295S (NT103)

    Plasmid

    #49066

    Purpose

    Expresses human NKCC1 C295S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C295S in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. HNF4A_P2-198

    Plasmid

    #31065

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

    Insert

    HNF4A P2 promoter (HNF4A Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    -198 to -1 of P2 HNF4A promoter

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. N-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58693

    Purpose

    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    

    Use

    AAV and CRISPR

    Tags

    3xFlag, GyrA Nsplit Intein, and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

    Plasmid

    #67980

    Purpose

    Cas9 activity reporter with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Anti-VAChT [N425/45]

    Plasmid

    #190326

    Purpose

    Mammalian Expression Plasmid of anti-VAChT (Rat). Derived from hybridoma N425/45.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-VAChT (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc18a3 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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