We narrowed to 44,105 results for: cha

1. pLX304-EZH1-Y727F

   Plasmid

   #203582

   Purpose

   Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.

   Depositor

   Abhishek Chakraborty, William Kaelin

   Article

   Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

   Insert

   EZH1 (EZH1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   changed Tyrosine 727 to Phenylalanine for partial…

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX304-EZH1-D745N

   Plasmid

   #203585

   Purpose

   Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.

   Depositor

   Abhishek Chakraborty, William Kaelin

   Article

   Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

   Insert

   EZH1 (EZH1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   changed Aspartic Acid 745 to Asparagine for parti…

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3

   Plasmid

   #206093

   Purpose

   Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   E314A, E664A, E1370A, E1658A

   Promoter

   CMV

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS

   Plasmid

   #206098

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E314A, E664A, E1370A, E1658A

   Promoter

   CMV/B-actin

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-puro-shRPS6

   Plasmid

   #125780

   Purpose

   constitutive expression of a short-hairpin RNA targeting human RPS6 (RNAi positive control)

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   shRPS6 (RPS6 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXPR_BRD003-sgCh2-2

   Plasmid

   #125767

   Purpose

   constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control)

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   sgCh2-2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXPR_BRD003-sgCh2-4

   Plasmid

   #125768

   Purpose

   constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control)

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   sgCh2-4
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLgw EcoDam-V5-ELK4

   Plasmid

   #98606

   Purpose

   Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK4 (Elk4 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   Dam (DNA adenine methyltransferase) and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMiR-CIP2A-miR-375-mutC&D

   Plasmid

   #53712

   Purpose

   Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

   Depositor

   Edward Chan

   Article

   Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

   Insert

   CIP2A (CIP2A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Mutation on miR-375 binding site C&D (refer t…

   Promoter

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pMiR-CIP2A-miR-375-mutE

    Plasmid

    #53710

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site E (refer to cita…

    Promoter

    Available Since

    July 11, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pMiR-CIP2A-miR-375-mutA&B

    Plasmid

    #53711

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site A&B (refer t…

    Promoter

    Available Since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pMiR-CIP2A-miR-375-mutD

    Plasmid

    #53709

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site D (refer to cita…

    Promoter

    Available Since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pMiR-CIP2A-miR-375-mutC

    Plasmid

    #53708

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site C (refer to cita…

    Promoter

    Available Since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pMiR-CIP2A-miR-375-mutB

    Plasmid

    #53707

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site B (refer to cita…

    Promoter

    Available Since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pMiR-CIP2A-miR-375-mutA

    Plasmid

    #53706

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site A (refer to cita…

    Promoter

    Available Since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pLX209-neo-active WRN

    Plasmid

    #125788

    Purpose

    constitutive, ectopic expression of human active WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-active WRN (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX209-neo-WRN K577M

    Plasmid

    #125790

    Purpose

    constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-WRN K577M (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    K577M (helicase-dead)

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX209-neo-WRN E84A

    Plasmid

    #125789

    Purpose

    constitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-WRN E84A (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    E84A (exonuclease-dead)

    Promoter

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLX209-neo-WRN E84A K577M

    Plasmid

    #125791

    Purpose

    constitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-WRN E84A/K577M (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    E84A/K577M (exonuclease/helicase-dead)

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid

    #232116

    Purpose

    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    BCKDK (BCKDK Human)
    

    Use

    Tags

    6 x His, Biotin acceptor peptide (BAP), Maltose-B…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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