We narrowed to 4,396 results for: erf
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Plasmid#159407PurposeConstitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.tet-LuxR-pr.LuxR-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialPromotertet and pLux promoterAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJUMP29[dCas9]-1A(lacZ)
Plasmid#126979PurposeLevel 1 vector. With lacZ as alternative cloning reporter.OriV 9 (pBBR322/ROP; medium copy number). Constitutive dCas9 in downstream secondary site.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationCas9 catalytically deadAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0078
Plasmid#117645PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.DepositorInsert[35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0808
Plasmid#91024PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_dead (D10A + H840A)
UseCRISPRMutationD10A, H840APromoterEC1.2Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0408
Plasmid#91010PurposeModule A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertAtCas9_dead (D10A + H840A)
UseCRISPRMutationD10A, H840APromoterEC1.2Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRi(v2)-Blast
Plasmid#170068PurposeExpresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistanceDepositorInsertKRAB-dCas9-MeCP2
UseCRISPR and LentiviralTags2A and 2xHA tagExpressionMammalianPromoterEFS-NSAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRE-dCas9-KRAB-MeCP2-BSD
Plasmid#140690PurposeInducible knockdown of gene expression in human cells for pooled scRNA-seq experimentsDepositorInsertCas9m4-KRAB-MeCP2
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTDH3-dCas9-Mxi1
Plasmid#46921PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoterDepositorInsertdCas9-Mxi1
UseCRISPRExpressionYeastPromoterTDH3Available SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB_tre_dCas9_KRAB
Plasmid#126030PurposePiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusionDepositorInsertsdCas9 fusion with KRAB
Hygromycin resistance
UseCRISPR; PiggybacExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterEF1 alpha and TREAvailable SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
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CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1
Plasmid#138418PurposeCAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1DepositorInsertdCas9
UseLentiviralTagsBioTAP-tagPromoterEF1aAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti_dCas9-KRAB-MeCP2
Plasmid#122205PurposeBipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.DepositorInsertdCas9-KRAB-MeCP2
UseLentiviralTagsKRAB-MeCP2ExpressionMammalianMutationCas9 (D10A, H840A)Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
TagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ABI1-dCas9
Plasmid#154474PurposeExpresses ABI1 fused to the N terminus of dCas9DepositorInsertABI1 (ABI1 Mustard Weed)
UseLentiviralExpressionMammalianMutationIncludes ABI1 aa 126-423, D143APromoterCMVAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-dCas9-ZIM3
Plasmid#240527PurposeExpresses MMLVgag–dCas9-ZIM3 for producing RENDER-dCas9-ZIM3DepositorInsertMMLVgag-dCas9-ZIM3
TagsFLAG, HAExpressionMammalianPromoterCMVAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_dCas9-XTEN-KRAB-p2A-mCherry
Plasmid#222107PurposeDonor vector for CRISPRi integration at AAVS1 with an mCherry fluorophoreDepositorInsertCRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)
UseAAV and CRISPRTagsHA and P2A-mCherryExpressionMammalianMutationD10A, H840AAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCB-1
Plasmid#210387PurposeAll-in-one cumate-based inducible CRISPRi plasmid for use in StreptomycesDepositorInsertsdCas9
CymR
sgRNA cassette
UseCRISPR; Integrative vector for use in streptomycesMutationBsaI-flanked RFP cassette inserted in place of sp…PromoterSP11 and SP30Available SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ223 (dAsCpf1-SRDX)
Plasmid#86209PurposeDeactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmidDepositorInsertdAsCpf1
UseGateway compatible cpf1 vectorsTagsNLS and SRDX repressor and NLSExpressionPlantMutationD908AAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only