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pAAV-short Cre-on G/W

Plasmid

#86949

Purpose

Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAV

Depositor

Joseph Dougherty, Rob Mitra

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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HOXD4 (murine) MSCV

Plasmid

#8549

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXD4 (Hoxd4 Mouse)

Use

Retroviral

Tags

His and T7

Expression

Mammalian

Mutation

contains T7 and HIS tags from pET; makes protein …

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA3_shRNA resistant PKA-Cα-mEGFP

Plasmid

#114143

Purpose

Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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mCHOP10(dLZ).pCDNA1

Plasmid

#21900

Depositor

David Ron

Article

Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

Insert

CHOP10 (Ddit3 Mouse)

Expression

Mammalian

Mutation

The coding region of the murine CHOP-10 cDNA was …

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only
HOXB7 (human) pCMV

Plasmid

#8539

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pDP1.2

Plasmid

#171092

Purpose

Expresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)

Depositor

Dario Palmieri

Article

Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

Insert

AAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1

Use

Synthetic Biology

Tags

Emerald, Geminin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

Plasmid

#163644

Purpose

Makes a lentivirus that expresses mNeonGreen and Cas9.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

mNeonGreen-P2A-Cas9

Use

Lentiviral

Tags

mNeonGreen-P2A

Promoter

EFS

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HOXB3 (human) HIS-tag pET

Plasmid

#8524

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB3 (HOXB3 Human)

Tags

His and T7

Expression

Bacterial

Mutation

Contains 5 additional amino acids before ATG from…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-Ro2

Plasmid

#24503

Depositor

Bruce Conklin

Article

Coward et al Proc Natl Acad Sci U S A. 1998 Jan 6;95(

Insert

RASSL Ro2 (OPRK1 Human)

Tags

FLAG and NLS from prolactin

Expression

Mammalian

Mutation

mutations in extracellular loops 2 (see comments …

Available Since

June 16, 2010

Availability

Academic Institutions and Nonprofits only
pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

Plasmid

#90011

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C S216A
NBirA*-14-3-3e

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Ser 216 to Ala

Promoter

CMV

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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HOXD4 (murine) HIS-tag pET

Plasmid

#8548

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXD4 (Hoxd4 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Western and by in vitro transcri…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
P63-sp64 Poly A

Plasmid

#59435

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 63

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
P29-sp64 Poly A

Plasmid

#59434

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 29

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
HOXB9 (murine) HIS-tag pET

Plasmid

#8541

Depositor

Corey Largman

Article

Chang et al Mol Cell Biol 1996 Apr;16(4):1734-45.

Insert

HOXB9 (Hoxb9 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

Missing the first 13 amino acids of HOXB9; makes …

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
P21-sp64 Poly A

Plasmid

#59433

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 21

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Rog

Plasmid

#24429

Depositor

Bruce Conklin

Article

Scearce-Levie et al BMC Biol. 2005 Feb 11;3:3. doi: 10.1186/

Insert

RASSL (OPRK1 Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

mutations in extracellular loops 2 (see comments …

Available Since

June 16, 2010

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

Plasmid

#216171

Purpose

Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-ATG13 (1-197)

Plasmid

#188074

Purpose

To make ATG101/ATG13 HORMA complex for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Expression

Mammalian

Mutation

codon-optimized ATG13 (1-197)

Promoter

CMV

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTripZ-B2

Plasmid

#163924

Purpose

For making a cell line that expresses doxycyline-inducible RNAi suppressor protein B2 for increasing titer of lentivirus with bidirectional promoters during virus production.

Depositor

John Engelhardt

Article

Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

Insert

Flock house virus B2 protein (FHVs1gp2 )

Use

Lentiviral

Expression

Mammalian

Mutation

WT

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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FUS_LC_charge

Plasmid

#139126

Purpose

express FUS LC with an hnRNPA2 LC-like charge patterning

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Expression

Bacterial

Mutation

Add R, K, D to make FUS LC have similar charge pa…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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