Skip to main content
Addgene
Showing: 7441 - 7480 of 10228 results

  1. pcDNA3-mCherry-TSC2(AA)

    Plasmid
    #192442
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    mCherry-TSC2(S939A,T1439A) (TSC2 Human)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192442-plasmid-map-sequence-id-379128

    Enlarge

  7. p168

    Plasmid
    #62618
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62618-plasmid-map-sequence-id-103064

    Enlarge

  10. pcDNA3-pmEMBer

    Plasmid
    #174441
    Purpose
    Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.
    Depositor
    Jin Zhang
    Article
    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1
    Insert
    pmEMBer
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    174441-plasmid-map-sequence-id-344972

    Enlarge

  13. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

    Enlarge

  14. RGD-sfGFP-I27-CC

    Plasmid
    #85392
    Purpose
    MTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    RGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85392-plasmid-map-sequence-id-172694

    Enlarge

  21. pRSF-G1mCNPRS

    Plasmid
    #174718
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    174718-plasmid-map-sequence-id-346623

    Enlarge

  25. pQZ210

    Plasmid
    #106414
    Purpose
    Co-expresses trans SNARE complex with pDC183 in E.coli
    Depositor
    Axel Brunger
    Article
    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d
    Inserts
    Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
    Syntaxin-1A fragment 191-256 (Stx1a Rat, Synthetic)
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Fragment 191-256 , Codon optimization and Fragmen…
    Promoter
    T7
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106414-plasmid-map-sequence-id-204167

    Enlarge

  37. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73196-plasmid-map-sequence-id-134659

    Enlarge

Showing: 7441 - 7480 of 10228 results