We narrowed to 11,439 results for: 112

1. pDONR221_SLC45A4

   Plasmid

   #132179

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC45A4 (SLC45A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pHH0103_LYN-1/1

   Plasmid

   #91536

   Purpose

   Protein expression and purification of human SH3 domain construct LYN-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   LYN-1/1 (LYN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-KLF12/pcDNA3.1-Neo

   Plasmid

   #81069

   Purpose

   Expression of epitope-tagged human KLF12 in mammalian cells.

   Depositor

   Julian Downward

   Article

   Godin-Heymann et al Oncogene. 2016 Jun 23;35(25):3324-34. do

   Insert

   FLAG-KLF12 (KLF12 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag

   Plasmid

   #185681

   Purpose

   Low-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Synthetic, Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Deletion of lumenal domain

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FXYD2-luc

   Plasmid

   #31056

   Depositor

   Gerhart Ryffel

   Article

   Adalat et al J Am Soc Nephrol. 2009 May . 20(5):1123-

   Insert

   FXYD2 (FXYD2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   FXYD2 promoter from -2382 to -1

   Promoter

   Available since

   Aug. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flrt1-Fc-His

   Plasmid

   #72073

   Purpose

   Expresses the extracellular region of the FLRT1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Flrt1 (Flrt1 Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag

   Plasmid

   #185684

   Purpose

   Low-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Synthetic, Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   K121Y

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX Lyn-SH2

   Plasmid

   #46452

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Lyn (LYN Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 106-213

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant

   Plasmid

   #149507

   Purpose

   Transient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Insert

   MFSD12 (MFSD12 Human)
   

   Use

   Transient expression

   Tags

   TEV-3XHA-3XFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized Sequence, p.LL256-257AA

   Promoter

   CMV

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. GPR45-Tango

    Plasmid

    #66360

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR45 (GPR45 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pPW3763 : CMVd3-HsIRE1dLKR-HaloTag

    Plasmid

    #185685

    Purpose

    Low-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Synthetic, Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Deletion of linker, kinase, and Rnase domains

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMX-IY LC3A

    Plasmid

    #89290

    Purpose

    For expression of the mammalian Atg8 protein LC3A without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    LC3A (MAP1LC3A Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. K15-TK/pGL3

    Plasmid

    #44267

    Depositor

    George Cotsarelis

    Article

    Ito et al Nat Med. 2005 Dec;11(12):1351-4. Epub 20

    Insert

    K15 promoter (-4.8) (Krt15 Mouse)
    

    Use

    Mouse Targeting; Thymidine kinase

    Tags

    HSV-1 TK

    Expression

    Mammalian

    Mutation

    Contains the murine K15 promoter (-4.8) fragment

    Promoter

    murine K15 promoter (-4.8) fragment

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pGL3-IRES-Lin28b-P3

    Plasmid

    #64794

    Purpose

    Luciferase expression driven by Lin28b promoter P3

    Depositor

    Joshua Mendell

    Article

    Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Lin28b promoter P3 (LIN28B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Pdpy-30-miniCAFE

    Plasmid

    #170115

    Purpose

    Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    MiniCAFE
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D8A, HNH-truncation (Δ495–609 aa) in CjCas9

    Promoter

    Dpy-30

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS-foxc1a-2a-nlstdTomato

    Plasmid

    #132970

    Purpose

    full-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Inserts

    tdTomato
    foxc1a (foxc1a Zebrafish)
    

    Use

    Tags

    NLS and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1a_HOXB6_P2A_Hygro_Barcode

    Plasmid

    #120454

    Purpose

    Barcoded lentiviral vector to express HOXB6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flrt1-AP-His

    Plasmid

    #71947

    Purpose

    Expresses the extracellular region of the FLRT1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Flrt1 (Flrt1 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-2T-TRR-C421-Y2404F

    Plasmid

    #182846

    Purpose

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRR 2011-2431 (trr Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP Kind2 S181E

    Plasmid

    #105312

    Purpose

    To study altered downstream functions of phospholylation-site mimetic kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S181E

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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