We narrowed to 173,051 results for: addgene
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Plasmid#107168Purposeexpresses V5-tagged miniTurbo in the yeast cytosolDepositorInsertminiTurbo (BirA mutant)
ExpressionYeastMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-GFP
Plasmid#78546PurposeFusion protein Cas9+GFPDepositorInsertGFP
UseLentiviralExpressionMammalianPromoterFusion protein with Cas9Available SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-EF1afull-H2B-mCherry-P2A-Fluc
Plasmid#207253PurposeLentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoterDepositorInsertH2B-mCherry-P2A-Fluc
UseLentiviralExpressionMammalianPromoterEF1alpha_fullAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ytdTomato-URA3
Plasmid#168054PurposeReplaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertytdTomato
TagsytdTomatoExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDM028
Plasmid#216809PurposeVector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.DepositorTypeEmpty backboneUseCRISPR; Fungal expressionExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2 sgSp1
Plasmid#174907PurposeSecond generation sgRNA against Sp1DepositorInsertSpecificity Protein 1 (SP1 Human)
ExpressionMammalianMutationMissense mutation to mutate PAM sequencePromoterEFS-NSAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-modified
Plasmid#84477Purposeguide RNA expression lentiviral constructDepositorInsertguide RNA
UseLentiviralExpressionMammalianPromotermU6Available SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
TurboID-V5_pRS415
Plasmid#107167Purposeexpresses V5-tagged TurboID in the yeast cytosolDepositorInsertTurboID (BirA mutant)
ExpressionYeastMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDM068
Plasmid#216811PurposeVector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.DepositorTypeEmpty backboneUseCRISPR; Fungal expressionExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymYPET-URA3
Plasmid#168056PurposeReplaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymYPET
TagsymYPETExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-GFP-IRES-CRE
Plasmid#48201Purposeretroviral vector encoding for GFP and Cre-recombinaseDepositorInsertseGFP
Cre
UseRetroviralExpressionMammalianPromoterCAG and linked by IRESAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNLS-VP16-NIR-Fb(718)
Plasmid#220745PurposeNLS-VP16-NIR-Fb(718) consrtuct expression in mammalian cells.DepositorInsertNIR-Fb(718)
UseAffinity Reagent/ AntibodyTagsNLS-VP16ExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Connexin43-GFP-APEX2
Plasmid#49385PurposeExpresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cellsDepositorInsertConnexin43 - EGFP - APEX2
ExpressionMammalianMutationK14D, W41F, E112K, A134P on soybean APX; improved…PromoterCMVAvailable SinceNov. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188-PrARG8-GFP::URA3
Plasmid#216512PurposeIntegrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrARG8-yeGFP
UseCre/Lox; IntegrativeExpressionYeastPromoterARG8Available SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB2197-PrGNP1-mK2-NatMX
Plasmid#216513PurposeIntegrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrGnp1-mKate2
UseCre/Lox; IntegrativeExpressionYeastPromoterGNP1Available SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDM030
Plasmid#216810PurposeVector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.DepositorTypeEmpty backboneUseCRISPR; Fungal expressionExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
P23_vanR_Ptec_mCherry
Plasmid#248114PurposeVanillate switch driving mCherry expressionDepositorInsertsmCherry red fluorescent protein
VanR repressor
ExpressionBacterialPromoterP23 and Ptec flanked by vanO operatorsAvailable SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymTq2-URA3
Plasmid#168057PurposeReplaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymTurquoise2
TagsymTurquoise2ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-mgSrtA
Plasmid#125792PurposeTransfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.DepositorInsertmgSrtA
ExpressionMammalianAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG2IG-Hmga1a
Plasmid#122293PurposeOverexpression for mouse Hmga1aDepositorInsertHmga1a (Hmga1 Mouse)
ExpressionMammalianAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only