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pCAG-SRGAP2A-FBAR-mRFP

Plasmid

#168502

Purpose

Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Insert

SRGAP2A-FBAR (SRGAP2 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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B52 + TIMELESS sgSTOP

Plasmid

#100713

Purpose

B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting TIMELESS (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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B52 + PARP4 sgSTOP

Plasmid

#100711

Purpose

B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting PARP4 (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-T0-eGFP-HROB

Plasmid

#135299

Purpose

Expresses eGFP-tagged HROB in mammalian cells

Depositor

Daniel Durocher

Article

Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

Insert

HROB (HROB Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pScalps_puro_mIL-2Rα RK

Plasmid

#59920

Purpose

Expresses mouse interleukin-2 receptor alpha chain mutated

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Mutation

changed sequence of the intracellular (C-terminal…

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
AcrIIC1X* (AcrIIC1X-mCherry chimera)

Plasmid

#128114

Purpose

Expresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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B52 + PIK3R1 sgSTOP

Plasmid

#100714

Purpose

B52 plasmid expressing PI3KR1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting PIK3R1 (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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SLC30A10-delta105-107

Plasmid

#65205

Purpose

Constitutive expression of SLC30A10 delta 105-107 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Leyva-Illades et al J Neurosci. 2014 Oct 15;34(42):14079-95.

Insert

SLC30A10 (SLC30A10 Human)

Tags

Flag

Expression

Mammalian

Mutation

delta 105-107 amino acids

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
p-OiRS3GG

Plasmid

#98589

Purpose

Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).

Depositor

Lydia Contreras

Article

Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552

Inserts

group I intron
PheS cassette to be selectively replaced by unique asRNA probes

Expression

Bacterial

Promoter

pBAD and pLtetO

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRS316-pTEF-Sc_IntAct-tCYC

Plasmid

#248980

Purpose

Low-copy centromeric plasmids which expresses Saccharomyces cerevisiae IntAct (actin internally tagged with ALFA tag between T229 and A230 amino acids)

Depositor

Saravanan Palani

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Sc_IntAct

Tags

ALFA tag

Expression

Yeast

Promoter

TEF

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pDUAL-pAct1-Sp_IntAct-tADH1

Plasmid

#250862

Purpose

Low-copy centromeric plasmids which expresses Schizosaccharomyces pombe IntAct (actin internally tagged with ALFA tag between T229 and A230 amino acids)

Depositor

Saravanan Palani

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Sp_IntAct

Tags

ALFA tag

Expression

Yeast

Promoter

TEF

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-4804

Plasmid

#249320

Purpose

Expresses Non-targeting sgRNA and contains the Repair Template to replace the KanR* on the genome of E. coli MG1655

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

nontargeting sgRNA

Expression

Bacterial

Mutation

N/A

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-5740

Plasmid

#249327

Purpose

Expresses sgRNA targeting the fcy gene in S. cerevisiae

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

sgRNA targeting fcy

Expression

Yeast

Mutation

N/A

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-9xmyc-WDR5

Plasmid

#236614

Purpose

Expresses human WDR5 in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

WDR5 (WDR5 Human)

Tags

9xmyc

Expression

Mammalian

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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PB-TA-ERN-WDR5-FLAG

Plasmid

#236721

Purpose

Expresses human WDR5 in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

WDR5 (WDR5 Human)

Use

Piggybac transposon

Tags

FLAG

Expression

Mammalian

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-AHPH

Plasmid

#187278

Purpose

Express mCherry-AHPH (RhoA biosensor)

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin and Pleckstrin homology domain (ANLN Human)

Tags

mCherry

Expression

Mammalian

Mutation

AHPH

Promoter

CMV

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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IL2-mCherry in pmCherryN1

Plasmid

#187283

Purpose

Express IL2-mCherry

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit (IL2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-3_FR[EF1α-copGFP]

Plasmid

#218326

Purpose

Donor plasmid of Tc1-3_FR Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pICOZ-MAG[EF1α-CD19 4-1BB]

Plasmid

#218329

Purpose

Donor plasmid of MAG Transposon with CD19 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pICOZ-MAG[EF1α-HER1 4-1BB]

Plasmid

#218331

Purpose

Donor plasmid of MAG Transposon with HER1 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

HER1 CAR mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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