165,075 results

1. EGFP-Peroxisomes-0

   Plasmid

   #54501

   Purpose

   Localization: Peroxisomes, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Type

   Empty backbone

   Tags

   EGFP and Peroximal Targeting Signal 1 (PTS1)

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCW57.1-FOXM1b

   Plasmid

   #68811

   Purpose

   Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells

   Depositor

   Adam Karpf

   Article

   Barger et al Oncotarget. 2015 Jul 16.

   Insert

   FOXM1b (FOXM1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRUPATH Triple Ga13

   Plasmid

   #196062

   Purpose

   Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha13-RLuc8 (GNA13 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP Cited2

   Plasmid

   #112246

   Purpose

   expression of EGFP-Cited2 in mammalian cells

   Depositor

   Simone Di Giovanni

   Insert

   Cited2 (CITED2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HTR2A-Tango

   Plasmid

   #66409

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   HTR2A (HTR2A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81

   Plasmid

   #100157

   Purpose

   Expresses MUS81

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   MUS81 (MUS81 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-Cre.zf1

   Plasmid

   #61391

   Purpose

   mRNA synthesis of Cre recombinase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Cre.zf1
   

   Use

   Zebrafish expression

   Promoter

   sp6

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-29b(+)_His-CBD-SpEndoH

   Plasmid

   #218196

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

   Depositor

   Florence Pojer

   Insert

   EndoH
   

   Tags

   tufA-His-CBD

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 45-313

   Promoter

   T7

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mNeonGreen-APEX3

   Plasmid

   #202590

   Purpose

   Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

   Depositor

   Reuben Harris

   Article

   Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

   Insert

   mNeonGreen-APEX3-V5 (APX1 )
   

   Use

   Retroviral

   Tags

   V5 epitope and mNeonGreen

   Expression

   Mammalian

   Mutation

   APEX2 L242A

   Promoter

   MESV LTR

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK036.TRE-Flpe-WPRE (Supernova)

    Plasmid

    #85007

    Purpose

    For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    NLS-FLPe-WPRE
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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