We narrowed to 9,777 results for: 158

1. nCas9-NG_P2A-mCherry

   Plasmid

   #219934

   Purpose

   Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   Sp-nCas9(D10A)-NG_P2A-mCherry
   

   Use

   CRISPR

   Tags

   SV40 bpNLS and SV40 bpNLS-P2A-mCherry

   Expression

   Mammalian

   Mutation

   nCas9(D10A)

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-HASAP1-ST

   Plasmid

   #138965

   Purpose

   chemigenetic voltage indicator, soma targeted

   Depositor

   Eric Schreiter

   Article

   Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

   Insert

   HASAP1-ST
   

   Use

   Tags

   somatic localization tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAWP78-PVCpnfΔpvc15_pvc12-HiBiT

   Plasmid

   #198309

   Purpose

   Negative control for payload loading assay; produces PVCs deficient for payload loader (pvc15)

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnfΔpvc15_pvc12-HiBiT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAWP78-PVCpnf_pvc13-Ad5DL491-492

   Plasmid

   #198290

   Purpose

   Produces PVCs retargeted with non-binding mutant of Ad5 knob

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-Ad5DL491-492
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. cpLOV2-V5

   Plasmid

   #168991

   Purpose

   circularly permuted LOV2 variant-V5

   Depositor

   Yubin Zhou

   Article

   He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

   Insert

   cpLOV2-V5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-HArcLight

   Plasmid

   #138326

   Purpose

   chemigenetic voltage indicator

   Depositor

   Eric Schreiter

   Article

   Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

   Insert

   HArcLight
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. enOgeuIscB with ωRNA-v2

   Plasmid

   #220939

   Purpose

   CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

   Inserts

   CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
   OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV-U6+27-Tornado-biRhoBAST

   Plasmid

   #196344

   Purpose

   Overexpression of circular biRhoBAST

   Depositor

   Murat Sunbul

   Article

   Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

   Insert

   Tornado-biRhoBAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHCMV-SS-antiSunTag-TM

   Plasmid

   #198306

   Purpose

   Displays anti-SunTag scFv from the surface of mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pHCMV-SS-antiSunTag-TM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA5/FRT-ChemoG1

    Plasmid

    #193806

    Purpose

    CMV overexpression of ChemoG1 in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG1
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    EGFP: A206K

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DL034:cFos::HaloTag-iPAK4

    Plasmid

    #177884

    Purpose

    HaloTag-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    cFos

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCR1326

    Plasmid

    #111131

    Purpose

    Expresses UBE2T.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    UBE2T (UBE2T Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-51b(+) ChemoG5

    Plasmid

    #193805

    Purpose

    Protein production of ChemoG5 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG5
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP:A206K, T225R; HT7: E143R, E147R, L271E

    Promoter

    T7

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TwinStrep-SUMO-Pdp1

    Plasmid

    #198320

    Purpose

    For affinity purification of Pdp1 payload

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    TwinStrep-SUMO-Pdp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CP2-TCC

    Plasmid

    #179679

    Purpose

    Complementary plasmid

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [sd2] T7 RNAP-TCC linker-degron
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBAD/HisD-TagRFP675

    Plasmid

    #44274

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

    Insert

    TagRFP675
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

    Promoter

    pBAD

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET47b-T7-dAsCas12f

    Plasmid

    #204641

    Purpose

    Expression of an inactive AsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    inactive AsCas12f
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D225A

    Promoter

    T7

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA5/FRT_COX8-CoA-Snifit(G41S)

    Plasmid

    #197946

    Purpose

    Expression of CoA-Snifit in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

    Insert

    Halo-GFP-ecPanK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PanK=PanK-G41S, D127A,L277W,F252Y

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LV anti-FITC-LaG16-R103A-T2A-BFP

    Plasmid

    #194935

    Purpose

    LV construct encoding R103A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTW194b63

    Plasmid

    #136930

    Purpose

    Directed evolution of SpCas10

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    npuC-gIII(C)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

    Plasmid

    #116963

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSTAP-seq_human_spike-in

    Plasmid

    #125152

    Purpose

    Empty vector to clone and measure the expression of promoter candidates (spike-in) by determining the abundance of transcripts originating from the candidate in human cells

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Type

    Empty backbone

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. py100-sgRNA_hVEGFA

    Plasmid

    #198282

    Purpose

    Expression of SpCas9 guide RNA specific for human VEGFA locus

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    py100-sgRNA_hVEGFA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TwinStrep-SUMO-Pnf

    Plasmid

    #198321

    Purpose

    For affinity purification of Pnf payload

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    TwinStrep-SUMO-Pnf
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAWP78-PVCpnf_Δpvc10

    Plasmid

    #198448

    Purpose

    Produces PVCs containing truncated spike tip protein (Pvc10).

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_Δpvc10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAWP78-PVCpnf_pvc13-MoonTag

    Plasmid

    #198299

    Purpose

    Produces PVCs retargeted with MoonTag

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-MoonTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FRB-polySH35

    Plasmid

    #175234

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-51b(+) ChemoG1

    Plasmid

    #193799

    Purpose

    Protein production of ChemoG1 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG1
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-51b(+) ChemoG2

    Plasmid

    #193800

    Purpose

    Protein production of ChemoG2 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG2
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP: A206K

    Promoter

    T7

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-51b(+) ChemoG3

    Plasmid

    #193801

    Purpose

    Protein production of ChemoG3 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG3
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP:A206K; HT7: L271E

    Promoter

    T7

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge