Addgene

We narrowed to 11,205 results for: SHA

Showing: 5611 - 5640 of 11205 results

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qTAG-AAVS1-Ef1a-Blast

Plasmid

#227267

Purpose

Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2 mCherry-Atl3

Plasmid

#86680

Purpose

Lentivirus to stably express fluorescent protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

ATL3 (ATL3 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-R2Tg_opt-3xFlag

Plasmid

#223251

Purpose

Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein_opt

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Z-lock cofilin

Plasmid

#141137

Purpose

controlling the actin-severing ability of cofilin with light

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Use

Tags

Expression

Mammalian

Mutation

mCherry, Zdk2(132F), Cofilin S3A, LOV WT

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP GLTP

Plasmid

#170746

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

Insert

GLTP (GLTP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-GFP

Plasmid

#39289

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

-1163 to +6 of nmt1 locus

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_H1_Gal4VP64

Plasmid

#169918

Purpose

Expression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch1-GL4VP64

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry_EHMT1_full length

Plasmid

#228810

Purpose

Expression of mouse EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

See Depositor Comments

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-R2Tg-3xFlag

Plasmid

#223250

Purpose

Mammalian expression of C-terminally Flag-tagged R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176273

Purpose

Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (sgTet) and BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G3BP1-S149A

Plasmid

#135998

Purpose

S149A G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S149A

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP(S65T)-His3MX6

Plasmid

#41598

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Yeast

Mutation

Promoter

TEF

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-GpNLuc

Plasmid

#208840

Purpose

Expresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

GpNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-DUX4-WT

Plasmid

#99282

Purpose

All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)

Depositor

Stephen Tapscott

Article

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (DUX4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Mito-ExRai-AMPKAR

Plasmid

#192448

Purpose

ExRai-AMPKAR targeted to the mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Mito-ExRai-AMPKAR

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pACS142

Plasmid

#204598

Purpose

Histone H3.3K9M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

human H3.3 K9M (H3-3A Human)

Use

Lentiviral

Tags

human histone H3.3 K9M - V5

Expression

Mammalian

Mutation

H3.3 K9M

Promoter

CMV

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AMPKAR(T/A)

Plasmid

#192447

Purpose

Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR(T/A)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Thr 6 in AMPK substrate mutated to Ala.

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Mutation

Promoter

U6 and hPGK

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-PSD95.FingR-eGFP-CCR5TC

Plasmid

#125693

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-YR-ICUE-mGold2t

Plasmid

#231790

Purpose

Visualization of cAMP dynamics using YR-ICUE-mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

YR-ICUE-mGold2t

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CMV BARK p2A mCherry

Plasmid

#117689

Purpose

CMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs peptide

Use

Tags

HA / FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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esmGFP-C10-pVTCB052

Plasmid

#231303

Purpose

Plasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coli

Depositor

Alexander Rives

Insert

esmGFP-C10

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAP2

Plasmid

#73211

Purpose

express Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)

Depositor

Luk Vandenberghe

Article

Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

Insert

AAP2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#126217

Purpose

Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSV human p85 alpha (HA tag)

Plasmid

#11499

Depositor

Ronald Kahn

Article

Ueki et al J Biol Chem. 2003 Nov 28. 278(48):48453-

Insert

p85 alpha (PIK3R1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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TRE3G-CTCF-WT-HA-MND-zeo

Plasmid

#232801

Purpose

Inducible over expression of wild type version of CTCF.

Depositor

Chunliang Li

Article

Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

Insert

CTCF (Ctcf Mouse)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.flex.H2B.GFP

Plasmid

#170368

Purpose

Cre-inducible AAV vector expressing nuclear GFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

flex H2B GFP

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pYFP-RPB1aAmr

Plasmid

#75284

Purpose

Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

Depositor

Xavier Darzacq, Robert Singer

Article

Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

Insert

POLR2A (POLR2A Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

N792D alpha-amanitin–resistant

Promoter

CMV

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1651-sgRNA(F+E)-sgGal4

Plasmid

#100549

Purpose

sgGal4-expressing vector modified from Addgene plasmid #51024

Depositor

Jian Xu

Article

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Insert

sgGal4

Use

CRISPR

Tags

sgRNA

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-Tubulin-C-18

Plasmid

#54292

Purpose

Localization: Microtubules, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Gao et al Cell. 2012 Dec 7;151(6):1370-85. doi: 10

Insert

Tubulin (TUBA1B Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits