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pEJS2700-CMV-Nme2-ABE8e-nt-E932R

Plasmid

#228721

Purpose

Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

N-terminal ABE8e with Nme2 nCas9 (D16A) E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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TREER-Actin

Plasmid

#208330

Purpose

TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER-Actin

Tags

TREER

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TOMM20-PuRRRE

Plasmid

#208329

Purpose

PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TOMM20-PuRRRE

Tags

PuRRRE

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry_gRNA

Plasmid

#80457

Purpose

gRNA expression vector with mCherry transfection control

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Inserts

sp Cas9 gRNA
mCherry

Use

CRISPR

Expression

Mammalian

Promoter

U6 and chicken β-actin promoter

Available Since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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modRNAc0-Cas9-2A-Puro

Plasmid

#172855

Purpose

IVT template plasmid for making modified Cas9Puro RNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

Cas9-2A-Puro

Use

Ivt template plasmid

Tags

2A-Puro

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS2712-CMV-eNme2-C-ABE8e-i1-L10

Plasmid

#228733

Purpose

Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid eNme-C nCas9 (D16A)-i1-L10

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2164_Nme2-ABE8e-i7

Plasmid

#201517

Purpose

Mammalian expression of domain inlaid Nme2-ABE8e-i7

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i7

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R

Plasmid

#228722

Purpose

Expresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

N-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2702-CMV-iNme2-ABE8e-nt

Plasmid

#228723

Purpose

Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

N-terminal ABE8e with iNme2 nCas9 (D16A)

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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modified pcAT7-Glo1

Plasmid

#160996

Purpose

Splicing reporter backbone

Depositor

Brenton Graveley

Article

Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

Insert

hemoglobin subunit beta (HBB Human)

Expression

Mammalian

Mutation

Repeated intron 1 with 40 nucelotide deletion

Promoter

CAG

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEJS2158_Nme2-ABE8e-i1

Plasmid

#201510

Purpose

Mammalian expression of domain inlaid Nme2-ABE8e-i1

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGSHU

Plasmid

#72244

Purpose

Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
hyg
I-SceI

Expression

Bacterial

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEJS2165_Nme2-ABE8e-i8

Plasmid

#201518

Purpose

Mammalian expression of domain inlaid Nme2-ABE8e-i8

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i8

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPS22 (pET23a-Cas10/Csm/csm2H6N)

Plasmid

#70038

Purpose

Bacterial expression of the S. epidermidis Cas10·Csm complex

Depositor

Luciano Marraffini

Article

Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

Insert

Cas10/Csm complex

Tags

6xHIS on N terminus of Csm2

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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modRNAc0-p53DD

Plasmid

#176902

Purpose

IVT template plasmid for making p53DD modRNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

p53DD

Use

Ivt template plasmid

Promoter

T7 promoter

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMSR0

Plasmid

#161984

Purpose

Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

Depositor

Olga Soutourina

Article

Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI661 pRPR1(TetO)-sgRNA

Plasmid

#139475

Purpose

Vector for tet-regulated sgRNA expression in yeast

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Insert

single guide RNA scaffold

Use

CRISPR

Expression

Yeast

Promoter

pRPR1(TetO)

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-Rab29

Plasmid

#186021

Purpose

2xHis-Rab29

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-Rab29 (RAB29 Human)

Expression

Bacterial

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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SaCas9_sgRNA_expression_in_pBluescript

Plasmid

#122090

Purpose

U6 driven SaCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS2191_Nme2-evoFERNY-nt

Plasmid

#201527

Purpose

Mammalian expression of N-terminally fused Nme2-evoFERNY-nt

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-evoFERNY-nt

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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