We narrowed to 5,565 results for: SPI

1. BPK1520 sgRPS3

   Plasmid

   #211526

   Purpose

   Facilitate knock-in of RPS3-Keima fusion protein

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   sgRPS3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPR-v2 hygro sgUCK1

   Plasmid

   #211524

   Purpose

   Deletes UCK1

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   sgUCK1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NK809

   Plasmid

   #196406

   Purpose

   Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta

   Depositor

   Nicole King

   Article

   Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

   Insert

   Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   N/A (synthetic)

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NK810

   Plasmid

   #196407

   Purpose

   Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

   Depositor

   Nicole King

   Article

   Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

   Insert

   Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   N/A (synthetic)

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NK811

   Plasmid

   #196408

   Purpose

   Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control

   Depositor

   Nicole King

   Article

   Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

   Insert

   Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   N/A (synthetic)

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPRC6-ProC

   Plasmid

   #198430

   Purpose

   mkate2 fluorescent reporter gene under control of promoter ProC. Used as a control plasmid in reporter gene assays.

   Depositor

   Eveline Peeters

   Article

   Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

   Insert

   mKate2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ProC and no promoter

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPRC6-ProD

   Plasmid

   #198431

   Purpose

   mkate2 fluorescent reporter gene under control of promoter ProD. Used as a control plasmid in reporter gene assays.

   Depositor

   Eveline Peeters

   Article

   Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

   Insert

   mKate2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ProD and no promoter

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPB EF1a CCR6-hLOV-TEVcs-Gal4

   Plasmid

   #173120

   Purpose

   Expresses CCR6 hLOV receiver construct in DIPG

   Depositor

   Alice Ting

   Article

   Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

   Insert

   CCR6-hLOV-TEVcs-Gal4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV CMV CCR6-eLOV-TEVcs-Gal4

   Plasmid

   #173113

   Purpose

   Expresses CCR6 receiver construct in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

   Insert

   CCR6-eLOV-TEVcs-tTA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGPN

    Plasmid

    #187482

    Purpose

    Expression vector - GFP

    Depositor

    Lewis Cantley

    Article

    Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

    Plasmid

    #183387

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    redesigned Caspase-4 (AA94-377) (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    ANR261ENP, D315H, M318K, T352K

    Promoter

    T7

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-IRES-GFP-mouse-Caspase-1-H340D

    Plasmid

    #183362

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 H340D (Casp1 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    H340D

    Promoter

    MESV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T

    Plasmid

    #183364

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1/4a (CASP4 Canis lupus familiaris)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    H342D_K345M_K379T

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSCV-IRES-GFP-dog-Caspase-1/4b

    Plasmid

    #183365

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1/4b (CASP4 Canis lupus familiaris)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSCV-IRES-GFP-dog-Caspase-1/4a

    Plasmid

    #183363

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1/4a (CASP4 Canis lupus familiaris)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4136457

    Plasmid

    #149537

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGBW-m4095418

    Plasmid

    #149525

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    d1-1088 d1203-1945

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046784

    Plasmid

    #145704

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046911

    Plasmid

    #145695

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046838

    Plasmid

    #145696

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 endoRNAse

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge