We narrowed to 9,933 results for: Uty

1. pET-28a-His6-SUMO-VAMP3

   Plasmid

   #135552

   Purpose

   Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP3
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pet15b-Endosomal Escape Control

   Plasmid

   #186552

   Purpose

   Negative Control confirming the need for a CPP peptide in mediating internalization

   Depositor

   Jean Gariepy

   Article

   Mohammed et al J Control Release. 2012 Nov 28;164(1):58

   Insert

   6H-ETA-GFP
   

   Use

   Tags

   6xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pet15b-Internalization and Endosomal Escape

   Plasmid

   #186551

   Purpose

   Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

   Depositor

   Jean Gariepy

   Article

   Mohammed et al J Control Release. 2012 Nov 28;164(1):58

   Insert

   10R-8His-ETA-EGFP
   

   Use

   Tags

   8xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Arp11 (1-417)

   Plasmid

   #51398

   Purpose

   expression of full-length mouse Arp11 in mammalian cells

   Depositor

   Trina Schroer

   Article

   Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

   Insert

   Arp11 (Actr10 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 5, 2014

   Availability

   Academic Institutions and Nonprofits only

5. FLAG-FLJ90652

   Plasmid

   #15359

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   FLJ90652 (INO80E Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMP71-tCD19-LLO-SIINFEKL

   Plasmid

   #174598

   Purpose

   expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and SIINFEKL epitopes

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   membrane bound CD19, LLO antigen, SIINFEKL (ovalbumin) antigen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-kcnk3a

   Plasmid

   #208985

   Purpose

   Riboprobe generation

   Depositor

   GuangJun Zhang

   Article

   Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

   Insert

   kcnk3a (kcnk3a Zebrafish)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAd-Sox2

   Plasmid

   #19767

   Depositor

   Konrad Hochedlinger

   Article

   Stadtfeld et al Science. 2008 Nov 7;322(5903):945-9. doi

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

9. p2H12Matry-blind

   Plasmid

   #221181

   Purpose

   cdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen-Matry(blind)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEM09

    Plasmid

    #135479

    Purpose

    Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunHsp70pr
    

    Use

    Tags

    GFP and hph

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Spun Hsp70pr

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-4T1-Claspin-FL-wt

    Plasmid

    #31621

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    Flag, GST, and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pMM9-MAPKK2-WT

    Plasmid

    #40805

    Depositor

    Natalie Ahn

    Article

    Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    MSV-LTR

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCDH-EF1a-tetR-T2A-iRFP

    Plasmid

    #176028

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    iRFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-VinculinI997A_TurboID_Venus

    Plasmid

    #200274

    Purpose

    Expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

    Depositor

    Brenton Hoffman

    Article

    Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

    Insert

    VinculinI997A_TurboID_Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    change isoleucine to alanine at 997 of vinculin

    Promoter

    T7

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHisAlix

    Plasmid

    #42576

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Bacterial

    Mutation

    silent mutation A1617G (numbering in ALIX gene)

    Promoter

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pFastBac(His10-SNAP-Csk)

    Plasmid

    #177895

    Purpose

    Baculo expression of His10 tag fused to SNAPtag and human Csk

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Csk (CSK Human)
    

    Use

    Tags

    10xHis-TEV-TVMV-SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIND-CLucZ

    Plasmid

    #53224

    Purpose

    Inducible expression of Cypridina luciferase and shRNAmir of interest

    Depositor

    Thorsten Stiewe

    Article

    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

    Inserts

    Cypridina luciferase
    non-silencing shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGK401red-hENT1

    Plasmid

    #166028

    Purpose

    Expresses human equilibrative nucleoside transporter 1 (hENT1)

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    human equilibrative nucleoside transporter 1 (SLC29A1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-ATG13

    Plasmid

    #171413

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ATG13 (ATG13 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. J23103_GB

    Plasmid

    #65991

    Purpose

    MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Constitutive promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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