169,232 results

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L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-ultraID-Blast

Plasmid

#207715

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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E228Q Vps4A-HA

Plasmid

#200087

Purpose

3x HA-VPS4A with E228Q dominant negative mutation in Dox-inducible pInducer20 vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

VPS4A (VPS4A Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

E228Q

Promoter

CMV

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPc3 K-RAS 4B

Plasmid

#236452

Purpose

transient overexpression of K-RAS 4B in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

K-RAS 4B (KRAS Human)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-RSV-tdTomato-2A-GCaMP6s

Plasmid

#80316

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Inserts

tdTomato
GCaMP6s

Use

Lentiviral

Promoter

RSV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-L-MYC

Plasmid

#139817

Purpose

doxycycline-inducible expression of Human L-MYC in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

L-MYC (MYCL Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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HA-GR

Plasmid

#215094

Purpose

Expresses HA-tagged Glucocorticoid Receptor in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

NR3C1 (NR3C1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN HAYFP-LOV24

Plasmid

#49570

Purpose

regulate protein stability with light

Depositor

Thomas Wandless

Article

Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

Insert

YFP-LOV24

Use

Retroviral

Tags

HA and YFP

Mutation

delete the last 3 aa from AsLOV2, with I532A muta…

Promoter

LTR

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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dPspCas13b-mutFTO

Plasmid

#223697

Purpose

Expressing fusion protein of dPspCas13b and inactive mutant of human FTO

Depositor

Chuan He

Article

Wei et al Science. 2022 May 27;376(6596):968-973.

Insert

dPspCas13b-GGS-mutFTO (FTO Human)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mAmetrine 1.1 FP

Plasmid

#52113

Purpose

a bicistronic IRES-mAtetrine containing retroviral vector

Depositor

Dario Vignali

Type

Empty backbone

Use

Retroviral

Tags

IRES-mAmetrine 1.1

Expression

Mammalian

Available Since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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