159,557 results

1. pSBtet-GB

   Plasmid

   #60504

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TCE & RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-ER-LAR-GECO1

   Plasmid

   #61244

   Purpose

   Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells

   Depositor

   Robert Campbell

   Article

   Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

   Insert

   LAR-GECO1
   

   Use

   Tags

   ER-retention sequence: KDEL and ER-targeting sequ…

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1: V51W/I113V/N35…

   Promoter

   CMV

   Available since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-iLACCO1.2

   Plasmid

   #218823

   Purpose

   Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

   Depositor

   Robert Campbell

   Article

   Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

   Insert

   iLACCO1.2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2/1

   Plasmid

   #112862

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Truncated P5

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pemiRFP670-N1

   Plasmid

   #136556

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   emiRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOS028: Mitycam calcium sensor (mitochondrial)

   Plasmid

   #163046

   Purpose

   Mitycam calcium sensor (mitochondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   Mitycam
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. NanoLuc-PM-Venus

   Plasmid

   #164786

   Purpose

   Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Oplophorus gracilirostris, Human)
   

   Use

   Tags

   NanoLuc and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

   Plasmid

   #92142

   Purpose

   Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   osTir1
   

   Use

   Mouse Targeting

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-U6-crRNA-empty

   Plasmid

   #134921

   Purpose

   Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   crRNA for CRISPR-Cas3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-Gsdmd

    Plasmid

    #80950

    Purpose

    Expresses Gsdmd in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

    Insert

    Gsdmd (Gsdmd Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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