We narrowed to 27,700 results for: CAL;
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Tom20-EYFP-miLID
Plasmid#210291Purposemitochondria marker and "anchor" for optogeneticsDepositorInsertTom20-EYFP-miLID
TagsEYFP and iLIDExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
mActA-mSspB-mCherry
Plasmid#210294PurposeActuAtor for iLID optogeneticsDepositorInsertmActA-,SspB-mCherry
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHC024 (∆SH)
Plasmid#226202PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).DepositorInsertHomology arms for soluble hydrogenase operon
UseSynthetic BiologyMutation∆hoxFUYHWI ∆hypA2B2F2Available SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC023 (∆MBH)
Plasmid#226201PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).DepositorInsertHomology arms for membrane bound hydrogenase operon
UseSynthetic BiologyMutation∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC022 (∆phcA)
Plasmid#226200PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.DepositorInsertHomology arms for PhcA
UseSynthetic BiologyAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC004 (∆phaCAB)
Plasmid#226198PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.DepositorInsertHomology arms for phaCAB
UseSynthetic BiologyAvailable SinceNov. 15, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC027 (∆pemK)
Plasmid#226204PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.DepositorInsertUpstream/downstream homology arms for addiction system toxinPemK.
UseSynthetic BiologyAvailable SinceNov. 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
8_T7-PH-mScarlet3
Plasmid#225935PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the PH membrane localisation signalDepositorInsertPH-mScarlet3
UseIn vitro transcriptionPromoterT7Available SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4636
Plasmid#215264PurposeModule for the expression of HISPS under the ed light regulated Etr8:CMVmin promoter and H3H under the copper inducible CBS:mDFR promoter and the constitutive expression of LUZ, CPH,EGFP and p19DepositorInsertEtr4:mDFR:HISPS-CBS4x:mDFR:H3HSF-LUZ-CPH-EGFP-p19
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4541
Plasmid#215260PurposeModule for the expression of the N. nambi HISPS gene under the regulation of the Red light-regulated Etr4:mDFR promoter and TNOS in and the constitutve expression of CPH, LUZ, H3H, EGFP and p19.DepositorInsertEtr4:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f L27A
Plasmid#204138PurposeProduction of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coliDepositorInsertjGCaMP8f L27A
TagsN-terminal His tagExpressionBacterialMutationLeucine 27 changed to AlaninePromoterT7Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only
