We narrowed to 79,973 results for: TRI

1. pKA-207I10

   Plasmid

   #79845

   Purpose

   Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

   Insert

   NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RpPpsR2 cysteine 439 changed to serine

   Promoter

   CMV

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1/Puro-CAG-ASAP1

   Plasmid

   #52519

   Purpose

   For mammalian expression of the fluorescent voltage sensor ASAP1

   Depositor

   Michael Lin

   Article

   St-Pierre et al Nat Neurosci. 2014 Apr 22. doi: 10.1038/

   Insert

   Accelerated Sensor of Action Potentials 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSUPER-scramble

   Plasmid

   #118349

   Purpose

   This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

   Depositor

   Lea Sistonen

   Article

   Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

   Insert

   scrambled shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLIC-SM-Myc

   Plasmid

   #111189

   Purpose

   High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

   Depositor

   Silvia Moreno

   Article

   Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

   Type

   Empty backbone

   Use

   Toxoplasma gondii expression

   Tags

   Spaghetti Monster Myc Tag

   Expression

   Mutation

   Promoter

   Endogenous

   Available since

   Aug. 2, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pCDH-SIRT6-H133Y

   Plasmid

   #102327

   Purpose

   Human SIRT6 H133Y for mammalian expression

   Depositor

   Hening Lin

   Article

   Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   changed histidine 133 to tyrosine

   Promoter

   CMV

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pWM_12x601_20bpLinker

   Plasmid

   #157787

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_20bp linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV.CAG.GLPLight1

   Plasmid

   #187467

   Purpose

   Expresses GLPLight1 in mammalian cells

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

   Insert

   GLPLight1
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. miReporter-PGK

   Plasmid

   #82477

   Purpose

   microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

   Insert

   H2B-mCherry and H2B-Citrine. HygroR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a MISTIC

   Plasmid

   #85999

   Purpose

   bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   N/A
   

   Use

   Tags

   HIS6 and MISTIC

   Expression

   Bacterial

   Mutation

   codon optimzed for bacterial expression

   Promoter

   T7

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMuLE_ENTR_TOP-iRFP_L5-L4

    Plasmid

    #113866

    Purpose

    Single pathway reporter; wnt promoter-driven expression of iRFP; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    TOP (wnt dependent promoter)
    iRFP713
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLOVE-miR302/367

    Plasmid

    #31310

    Depositor

    Ed Morrisey

    Article

    Anokye-Danso et al Cell Stem Cell. 2011 Apr 8. 8(4):376-88.

    Insert

    mmu-miR302/367
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

12. Fra-2

    Plasmid

    #187907

    Purpose

    Expresses Fra-2 in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    Fra2 (Fosl2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RA-NES

    Plasmid

    #61019

    Purpose

    ddRFP A-NES

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A
    

    Use

    Tags

    HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPIPL12-FnCas12a

    Plasmid

    #199563

    Purpose

    Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

    Depositor

    Philippe Lambin

    Article

    Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

    Insert

    Fncas12a
    

    Use

    CRISPR and Synthetic Biology; Tetracycline induci…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    COtetR-PIPL12

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-HA-Ebp1

    Plasmid

    #67792

    Purpose

    Mammalian expression of Ebp1 tagged with HA

    Depositor

    Stephen Goff

    Article

    Wang et al J Virol. 2014 Feb;88(3):1825-9. doi: 10.

    Insert

    Ebp1 (PA2G4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

16. ArcLight-S249

    Plasmid

    #36855

    Depositor

    Vincent Pieribone

    Article

    Jin et al Neuron. 2012 Sep 6;75(5):779-85.

    Insert

    fluorescent protein voltage sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    simian CMV

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-Akt

    Plasmid

    #39531

    Depositor

    Julian Downward

    Article

    Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

    Insert

    Akt (Akt1 Mouse)
    

    Use

    Tags

    6G linker and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

18. FUGW-UBC-EGFP-2A-BoNT-B (LC)

    Plasmid

    #185722

    Purpose

    Lentiviral expression of GFP and botulinum neurotoxin type B light chain from UbC promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    botulinum neurotoxin type B light chain
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET12a-PAPI

    Plasmid

    #19961

    Depositor

    Jonathan Tait

    Article

    Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

    Insert

    Annexin V (ANXA5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMD1 anti-GFP nanobody-Tn5

    Plasmid

    #215567

    Purpose

    Expresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tag

    Depositor

    Isabelle Mansuy

    Article

    Maria A. et al bioRxiv 2024.07.12.603224

    Insert

    anti-GFP nanobody-Tn5 fusion
    

    Use

    Tags

    3xFlag and intein-CBD

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge