We narrowed to 4,886 results for: phy

1. pGEX-4T-1-DGCR8-2xdsRBD(+6)

   Plasmid

   #171542

   Purpose

   Encodes a DGCR8 fragment extending from E502 to the C-terminus

   Depositor

   Yan Zeng

   Article

   Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   DGCR8 (DGCR8 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   a six amino acid_TRLTEG_insertion in the L1 region

   Promoter

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUMO ACD11

   Plasmid

   #170738

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

   Insert

   acd11 (ACD11 Mustard Weed)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CBSH3+ shRNA-2

   Plasmid

   #160068

   Purpose

   Knock Down SH3+ Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CBSH3+ shRNA-1

   Plasmid

   #160066

   Purpose

   Knock Down SH3+ Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GS2.1/EC

   Plasmid

   #132568

   Purpose

   sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).

   Depositor

   Mathias Pribil

   Article

   Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

   Insert

   egg cell promoter, Cas9-mApple, pds3 sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   A. thaliana egg cell promoter

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmApple-EGFP-HRas

   Plasmid

   #133854

   Purpose

   Mammalian expression of HRas N-terminally tagged with mApple and EGFP

   Depositor

   Dave Piston

   Article

   Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

   Insert

   HRas (HRAS Human)
   

   Use

   Tags

   EGFP and mApple

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMinDis.X513-iNicSnFR3b-(V7)-ER

   Plasmid

   #125123

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

   Depositor

   Henry Lester

   Article

   Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

   Insert

   iNicSnFR3a-(V7)-ER
   

   Use

   Tags

   Fusion protein -Endoplasmic retention motif

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHHM.X513-iNicSnFR3a-(CC93)

   Plasmid

   #124880

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

   Depositor

   Henry Lester

   Article

   Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

   Insert

   iNicSnFR3a-(CC93)
   

   Use

   Tags

   HA Tag and His Tag

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   T7

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHHM.X513-iNicSnFR2-(CC90)

   Plasmid

   #124836

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

   Depositor

   Henry Lester

   Article

   Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

   Insert

   iNicSnFR2-(CC90)
   

   Use

   Tags

   HA tags and His Tag

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   T7

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVL43 - pNST3>>

    Plasmid

    #116009

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pNST3:GR-LhG4:tNST3:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Use

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVL42 - pLTP1>>

    Plasmid

    #116008

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pLTP1:GR-LhG4:tLT1:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Use

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCHKU17.1-2.2

    Plasmid

    #115530

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)mGAT1-565-YFP-566CT

    Plasmid

    #41677

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; YFP inserted d…

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(+)mGAT1-570-CFP-571CT

    Plasmid

    #41680

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” CFP A206K mutation; CFP inserted d…

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)mGAT1-570-YFP-571CT

    Plasmid

    #41679

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; YFP inserted d…

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)mGAT1-565-CFP-566CT

    Plasmid

    #41678

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” CFP A206K mutation; CFP inserted d…

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSP64-hERG1a S631A

    Plasmid

    #53058

    Purpose

    Expresses alpha subunit of S631A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Zou et al J Physiol. 1998 May 15;509 ( Pt 1):129-3

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    changed Serine 631 to Alanine

    Promoter

    SP6

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)mGAT1CFP45

    Plasmid

    #41676

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” CFP A206K mutation; 45 C-terminal-…

    Promoter

    CMV

    Available since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)mGAT1YFP45

    Plasmid

    #41675

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; 45 C-terminal-…

    Promoter

    CMV

    Available since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. integrin beta6 777T pBS

    Plasmid

    #13581

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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