3,614 results

1. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. dmBACCS2NS-IRES-dOrai-IRES-GFP

   Plasmid

   #72895

   Purpose

   Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2NS-IRES-dOrai-IRES-GFP
   

   Use

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-EGFP-DmFMR1_B

   Plasmid

   #145920

   Purpose

   Insect Expression of DmFMR1

   Depositor

   Elisa Izaurralde

   Insert

   DmFMR1 (Fmr1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Two amino acid mutations (T214I and N501S) and th…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

   Plasmid

   #172065

   Purpose

   Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   mTagBFP2(11x7)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pCoHygro L16_shRNA of spliced Copia

   Plasmid

   #71389

   Purpose

   knock down expression of spliced Copia in Drosophila melanogaster

   Depositor

   Timothy Nilsen

   Insert

   Copia
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUASTLOTattB_eGFP::Dpp

   Plasmid

   #163702

   Purpose

   GFP-tagged version of Dpp (Decapentaplegic) under control of UAS and LexO enhancers

   Depositor

   Markus Affolter

   Article

   Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

   Insert

   decapentaplegic (dpp Fly)
   

   Use

   Cre/Lox

   Tags

   Tagged with eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PRE-Hsp70BbCas9_1.2

   Plasmid

   #190796

   Purpose

   gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-EGFP-DmRRP40

   Plasmid

   #65797

   Purpose

   expresses EGFP-tagged RRP40 in Drosophila cells

   Depositor

   Elisa Izaurralde

   Insert

   RRP40 (Rrp40 Fly)
   

   Use

   Drosophila expression

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Actin 5C

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pB1H2w2-mutOdd

   Plasmid

   #18044

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

   Insert

   mutant Odd Skipped
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A mutant version of the zinc finger protein Odd S…

   Promoter

   Available since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-6P-1-DmPABPC1_J

    Plasmid

    #146647

    Purpose

    Bacterial Expression of DmPABPC1

    Depositor

    Elisa Izaurralde

    Insert

    DmPABPC1 (pAbp Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G26S relative to NM_166265.2/NP_725750.1

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge