1,878 results

1. pMLS262

   Plasmid

   #73718

   Purpose

   C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Psnt-1::2xNLS-FLP-D5
   

   Expression

   Worm

   Mutation

   G5D

   Promoter

   Psnt-1

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. optGluCl alpha EYFP

   Plasmid

   #15104

   Depositor

   Henry Lester

   Article

   Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

   Insert

   GluCl alpha (glc-1 Nematode)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Codon optimized cDNA

   Available Since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTK73.ChrII_ttTi5605.sgRNA

   Plasmid

   #194058

   Purpose

   sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   ChrII_ttTi5605 sgRNA
   

   Expression

   Worm

   Promoter

   U6

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTK73.ChrV_oxTi365.gRNA

   Plasmid

   #194052

   Purpose

   sgRNA targeting ChrV_oxTi365 locus of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   ChrV_oxTi365 sgRNA
   

   Expression

   Worm

   Promoter

   U6

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSDH61

   Plasmid

   #139033

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   epdz (no STOP codon)
   

   Expression

   Worm

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

   Plasmid

   #159811

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBT3024

   Plasmid

   #122542

   Purpose

   Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2

   Depositor

   Gary Silverman

   Article

   Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

   Insert

   Pnhx-2_mt::CemOrange2
   

   Tags

   MLSLRQSIRFFKPATRTLCSSRTLL

   Expression

   Bacterial

   Promoter

   Pnhx-2

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCM1.35

   Plasmid

   #17248

   Depositor

   Geraldine Seydoux

   Article

   Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

   Insert

   GFP:H2B from pKS111-His in pDONR201
   

   Use

   Gateway

   Available Since

   Feb. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT3007

   Plasmid

   #122538

   Purpose

   Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a early endosome localized gene (rab-5) under the intestinal specific promoter nhx-2

   Depositor

   Gary Silverman

   Article

   Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

   Insert

   Pnhx-2_CemOrange2::rab-5 (rab-5 Synthetic, Nematode)
   

   Expression

   Bacterial

   Promoter

   Pnhx-2

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCM5.47

    Plasmid

    #17254

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    pie-1 3'utr in pDONRP2RP3
    

    Use

    Gateway

    Available Since

    Feb. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSA101

    Plasmid

    #59787

    Purpose

    drives ZIF-1 protein in C. elegans neurons

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    rab-3 promoter
    zif-1 coding (zif-1 Nematode)
    unc-54 3' UTR
    

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJA269

    Plasmid

    #21544

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    phsp16.41::GFP-F64LS65T::(Gly)5Ala
    

    Use

    Multisite gateway

    Available Since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSM03

    Plasmid

    #139043

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    dendra2 (includes STOP codon)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ150-Cas9(dpiRNA)

    Plasmid

    #107940

    Purpose

    eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    Cas9(dpiRNA)
    

    Expression

    Worm

    Promoter

    eft-3

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSO221 Phis-72 BirA GFP

    Plasmid

    #45100

    Depositor

    Steven Henikoff

    Article

    Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

    Inserts

    BirA::GFP
    unc-119 (unc-119 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    his-72 and unc-119

    Available Since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBT3037

    Plasmid

    #122544

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_sqst-1::CemOrange2 (T12G3.1 Synthetic, Nematode)
    

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. BSP188

    Plasmid

    #110917

    Purpose

    For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    unc54 Terminator
    

    Use

    Synthetic Biology; Yeast homologous recombination…

    Expression

    Worm

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFJ1201

    Plasmid

    #44483

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, unc-119, peel-1, MCS
    

    Use

    Multiple cloning site vector

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSH9

    Plasmid

    #90328

    Purpose

    Expresses the set-2.a cDNA isoform in the C. elegans intestine

    Depositor

    Anne Brunet

    Article

    Han et al Nature. 2017 Apr 13;544(7649):185-190. d

    Insert

    Pges-1::set-2a
    

    Expression

    Worm

    Promoter

    ges-1

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

    Plasmid

    #47542

    Purpose

    engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

    Depositor

    Henry Lester

    Article

    Frazier et al J Biol Chem. 2013 May 29.

    Insert

    Glutamate-gated chloride channels
    

    Expression

    Mammalian

    Mutation

    retention motif in ER (starting at R340) changed …

    Promoter

    CMV

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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