1,878 results
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Plasmid#198818PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kineticsDepositorInsert15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
ExpressionWormAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCM5.47
Plasmid#17254DepositorInsertpie-1 3'utr in pDONRP2RP3
UseGatewayAvailable SinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMLS262
Plasmid#73718PurposeC. elegans Pan-neuronal 2xNLS-FLP-D5 expression vectorDepositorInsertPsnt-1::2xNLS-FLP-D5
ExpressionWormMutationG5DPromoterPsnt-1Available SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEY52
Plasmid#191055Purposeodr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KG#203
Plasmid#110880PurposeExpresses the C. elegans pde-4 (isoform d) cDNA pan-neuronallyDepositorInsertsrab-3 promoter
pde-4d (isoform d) cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
ExpressionBacterialAvailable SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS1-gpa-2P::his-24::Cerulean
Plasmid#124337PurposeMulticolor Worms for Neuronal IDDepositorInsertgpa-2, Cerulean (gpa-2 Nematode)
ExpressionWormAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3014
Plasmid#122540PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a late endosome localized gene (rab-7) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH4542
Plasmid#200779PurposePtwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptuneDepositorInsertGCaMP6
TagsmNeptuneExpressionWormPromoterPtwk-40Available SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDSP32
Plasmid#199374PurposeEncodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
ExpressionBacterialMutationno mutations, except added subcellular localizati…Available SinceApril 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
Plasmid#159811PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-Cas9(dpiRNA)
Plasmid#107940Purposeeft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertCas9(dpiRNA)
ExpressionWormPromotereft-3Available SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJA269
Plasmid#21544DepositorInsertphsp16.41::GFP-F64LS65T::(Gly)5Ala
UseMultisite gatewayAvailable SinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSO221 Phis-72 BirA GFP
Plasmid#45100DepositorAvailable SinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSDH61
Plasmid#139033PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette systemDepositorInsertepdz (no STOP codon)
ExpressionWormAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLS2965
Plasmid#122534PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUA97
Plasmid#69340PurposeIt encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategyDepositorInsertceBFP SEC 3xFlag
Tags3xFlag and ceBFP (mTagBFP)ExpressionWormMutationsqt-1(e1350)Available SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK73.ChrII_ttTi5605.sgRNA
Plasmid#194058PurposesgRNA targeting ChrII_ttTi5605 locus of the C. elegans genomeDepositorInsertChrII_ttTi5605 sgRNA
ExpressionWormPromoterU6Available SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTK73.ChrV_oxTi365.gRNA
Plasmid#194052PurposesgRNA targeting ChrV_oxTi365 locus of the C. elegans genomeDepositorInsertChrV_oxTi365 sgRNA
ExpressionWormPromoterU6Available SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA101
Plasmid#59787Purposedrives ZIF-1 protein in C. elegans neuronsDepositorInsertsAvailable SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDD288
Plasmid#137017PurposemNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmNG-GLO^SEC^3xFlag + ccdB
UseCRISPR and Cre/LoxTags3xFlag and C. elegans GLO-optimized mNGExpressionWormAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only