We narrowed to 4,504 results for: pcr

1. pCR4-Dact1(1250-1601)

   Plasmid

   #52046

   Purpose

   To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.

   Depositor

   Ben Cheyette

   Article

   Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.

   Insert

   Dact1(1250-1601) (Dact1 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCRISPR-Cas9/CD4-TK2

   Plasmid

   #104397

   Purpose

   The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.

   Depositor

   Jeronimo Blanco

   Article

   Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39

   Insert

   TK2 (TK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCR-U6-gRNA-miniCMV-TdTomato

   Plasmid

   #192656

   Purpose

   A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   TdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, miniCMV

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCR3-Flag-AuroraB KD (K106R)

   Plasmid

   #108488

   Purpose

   expression of Flag-AuroraB kinase death (K106R)

   Depositor

   Susanne Lens

   Article

   Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

   Insert

   AuroraB (AURKB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Kinase death K106A

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBS537 tet-hCMV-GFPcre

   Plasmid

   #11960

   Depositor

   Brian Sauer

   Article

   Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

   Insert

   GFP-cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pCRII-TOPO GFR alpha 1

   Plasmid

   #119898

   Purpose

   Mouse GFR alpha 1 In situ hybridization probe

   Depositor

   Wenqin Luo, David Ginty

   Article

   Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

   Insert

   GDNF family receptor alpha-1 precursor (Gfra1 Mouse)
   

   Use

   In situ hybridization probe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PCRII-TOPO-ZFERV-ENV

   Plasmid

   #22399

   Depositor

   Lisa Steiner

   Insert

   ZFERV env gene (env Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pCRII-Sema4a-ISH-2kb

   Plasmid

   #68029

   Purpose

   Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

   Depositor

   Roland Friedel

   Article

   Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

   Insert

   Semaphorin-4A (Sema4a Mouse)
   

   Use

   Mrna in situ hybridization probe

   Tags

   Expression

   Mutation

   a 2kb cDNA fragment for ISH probe synthesis

   Promoter

   Available since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRII-Plxnb2-ISH-2kb

   Plasmid

   #68036

   Purpose

   Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

   Depositor

   Roland Friedel

   Article

   Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

   Insert

   Plexin-B2 (Plxnb2 Mouse)
   

   Use

   Mrna in situ hybridization probe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRII-Sema4c-ISH-2kb

    Plasmid

    #68031

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4C (Sema4c Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRII-Topo U6-SpAi9L-U6SpDMDL

    Plasmid

    #78608

    Purpose

    Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9L-U6SpDMDL
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pCRII-Topo U6-SpAi9R-U6-SpDMDR

    Plasmid

    #78609

    Purpose

    Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9R-U6-SpDMDR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pCRII-Sema4d-ISH-2kb

    Plasmid

    #68032

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4D (Sema4d Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRII-Plxnb1-ISH-2kb

    Plasmid

    #68035

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B1 (Plxnb1 Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBS596 tet-hCMV-GFPcre

    Plasmid

    #11961

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    GFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

16. (G38) pCRII-Wnt10b/b-globin

    Plasmid

    #25877

    Depositor

    Ormond MacDougald

    Article

    Longo et al J Biol Chem. 2004 Aug 20. 279(34):35503-

    Insert

    Wnt10b / b-globin (Wnt10b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pCRII-Plxnb3-ISH-2kb

    Plasmid

    #68037

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B3 (Plxnb3 Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRII-Sema4g-ISH-2kb

    Plasmid

    #68034

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4G (Sema4g Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXTK011 [Marker_SpcR-StrR-aadA_PartType4]

    Plasmid

    #229248

    Purpose

    XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_SpcR-StrR-aadA_PartType4

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Insert

    SpcR-StrR-aadA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXTK004 [Marker_SpcR-StrR-aadA_PartType2]

    Plasmid

    #229241

    Purpose

    XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_SpcR-StrR-aadA_PartType2

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Insert

    SpcR-StrR-aadA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCRISPEY-GAL-Hyg-ADE2

    Plasmid

    #232104

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCRISPEY-GAL-Kan-ADE2

    Plasmid

    #232105

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCRII-mogat3b in situ probe

    Plasmid

    #232136

    Purpose

    zebrafish mogat3b anti-sense in situ probe, linearize with BamHI, T7 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    mogat3b
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcRII-mogat3a in situ probe

    Plasmid

    #232135

    Purpose

    zebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    mogat3a
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCRII-dgat2 in situ probe

    Plasmid

    #232134

    Purpose

    zebrafish dgat2 anti-sense in situ probe, linearize with BamHI, T7 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    dgat2 (dgat2 Zebrafish)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7

    Plasmid

    #196846

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 2xPP7

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b

    Plasmid

    #196847

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 1xMS2

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCR 2.1-TOPO Fzd6HA

    Plasmid

    #208363

    Purpose

    Extracellular HA-tagged Fzd6 (mouse)

    Depositor

    Maureen Cetera, Danelle Devenport

    Article

    Cetera et al Development. 2023 Aug 25:dev.202078. doi

    Insert

    Fzd6 (Fzd6 Mouse)
    

    Use

    Cloning vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCR-U6-gRNA-TRE3G-Nluc

    Plasmid

    #192659

    Purpose

    A plasmid encoding TRE3G sgRNA and TRE3G-promoter-Nluc

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Nluc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, TRE3G

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCR-U6-gRNA-miniCMV-Nluc

    Plasmid

    #192657

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Nluc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, miniCMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge