Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 772 results for: tev cleavage

Showing: 721 - 760 of 772 results

  1. hTln1-R1R2-17b

    Plasmid
    #191427
    Purpose
    Expresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteria
    Depositor
    Ben Goult
    Article
    Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901
    Insert
    Human Talin1 R1R2 domains (TLN1 Human)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    contains 17AA insertion from inclusion of exon 17b
    Promoter
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    191427-plasmid-map-sequence-id-380511

    Enlarge

  5. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Article
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
    Covid-SARS2 Nsp10
    Use
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159613-plasmid-map-sequence-id-311056

    Enlarge

  8. pFBOH-MHL_SHKBP1:182-585

    Plasmid
    #210935
    Purpose
    Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    SHKBP1:T182-L585 (SHKBP1 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    Q507L natural variant (VAR_036714)
    Promoter
    Polyhedrin
    Available since
    March 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210935-plasmid-map-sequence-id-426241

    Enlarge

  16. pFBOH-MHL_WDR13:142-485

    Plasmid
    #210924
    Purpose
    Insect expression of human WDR13 fragment A142 to K485. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR13:A142-K485 (WDR13 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    H325R natural variant (VAR_060284)
    Promoter
    Polyhedrin
    Available since
    March 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210924-plasmid-map-sequence-id-426206

    Enlarge

  21. pFBOH-MHL_WDR12:90-417

    Plasmid
    #210903
    Purpose
    Insect expression of human WDR12 fragment T90 to T417. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR12:T90-T417 (WDR12 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    introduced mutations F99A K131A R319S
    Promoter
    Polyhedrin
    Available since
    March 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210903-plasmid-map-sequence-id-426133

    Enlarge

  29. pNICBio2_WDR5:2-334

    Plasmid
    #211027
    Purpose
    bacterial expression of human WDR5 fragment A2 to C334. N-terminal 10X His tag with TEV cleavage and C-terminal biotin acceptor peptide tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR5:A2-C334 (WDR5 Human)
    Use
    Tags
    MHHHHHHHHHHDLGTENLYFQS and SSKGGYGLNDIFEAQKIEWHE
    Expression
    Bacterial
    Mutation
    wild type
    Promoter
    T7
    Available since
    April 4, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    211027-plasmid-map-sequence-id-426277

    Enlarge

Showing: 721 - 760 of 772 results