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M4753 KanMX::ADE2 Disruptor Converter

Plasmid

#51683

Purpose

Yeast marker swap plasmid for converting KanMX3 to ADE2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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XE70 NLS Beta-catenin GFP in CS2+

Plasmid

#16838

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

NLS beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
NK66

Plasmid

#176606

Purpose

To measure Syk intensity in response to ligand clustering using DNA origami

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

Syk-BFP (Syk Mouse, Synthetic)

Use

Lentiviral

Expression

Mammalian

Mutation

n/a

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huHkCas12a

Plasmid

#174683

Purpose

Expresses 6xHis-MBP tagged HkCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huHkCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSARSF505

Plasmid

#46832

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

GFPN-NpuDnaE_intein-GFP_C

Tags

GFP_C and H6-GFP_N

Expression

Bacterial

Mutation

stemmer GFP with superfolder mutations S30R and Y…

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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TOPO mKO2

Plasmid

#68442

Purpose

TOPO construct expressing mKusabira-Orange2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mKusabira-Orange2

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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XE71 NES Beta-catenin GFP in CS2+

Plasmid

#16837

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

NES beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pNIR-Fb4EIG

Plasmid

#184683

Purpose

NIR-Fb to E.coli dihydrofolate reductase

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb4EIG

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B

Plasmid

#21683

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

maternal expression at 31B (me31B Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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TOPO Venus

Plasmid

#68493

Purpose

TOPO construct expressing Venus for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

split Venus

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRC04

Plasmid

#114683

Purpose

FRB-TEV-N

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

FRB-TEV-N

Tags

MBP

Expression

Bacterial

Promoter

Tac Promoter

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEBB FLAG XIAP D148A

Plasmid

#25683

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

FLAG

Expression

Mammalian

Mutation

D148A

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
XE86 TM Delta N Beta-catenin GFP

Plasmid

#16833

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

TM Delta N beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
YCe1901 HC_Kan_PGKpA_p22

Plasmid

#100683

Purpose

part designed to occupy position 22 of EMMA. Functional category: polyadenylation signal sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

PGKpA

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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XE75 Beta-catenin Delta N-9 GFP

Plasmid

#16831

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

beta-catenin Delta N - 9 GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
TOPO CloverCP

Plasmid

#68444

Purpose

TOPO construct expressing CloverCP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

CloverCP

Use

Gmap

Tags

"CP" degradation domain

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO mT2

Plasmid

#68491

Purpose

TOPO construct expressing mT2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mTurquoise2

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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XE73 TM Beta-catenin 1-myc in CS2+

Plasmid

#16834

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

TM beta-catenin 1-myc (ctnnb1.L Frog)

Use

Xenopus expression

Tags

c-myc

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pMS161

Plasmid

#215683

Purpose

Guide only plasmid targeting chrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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vYM015_phiC31-nTRE-Ins(low)-pEF1s(orig)-H2b-Citrine

Plasmid

#197683

Purpose

"Insert(low)-pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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