We narrowed to 430 results for: AAVS1

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pAdSh.U6.gRNAS1

Plasmid

#58252

Purpose

U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

U6.gRNAS1 cassette

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-ZFN_L-HiBiT

Plasmid

#198319

Purpose

Loads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-ZFN_L-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTS2163

Plasmid

#169632

Purpose

Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

OTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3

Expression

Mammalian

Promoter

tetO7-PhCMVmin / PPGK

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKD2.0

Plasmid

#220536

Purpose

AAVS1-targeting vector designed for the iPS2-seq platform to express a single-copy, tetracycline-inducible shRNA in human pluripotent stem cells

Depositor

Alessandro Bertero

Article

Balmas et al Mol Syst Biol. 2025 Dec 10. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Neo_CAG

Plasmid

#220537

Purpose

AAVS1-targeting backbone used as a filler plasmid to co-deliver during nucleofection with pAAV-Puro_siKD2.0 (Plasmid #220536) cloned with shRNAs

Depositor

Alessandro Bertero

Article

Balmas et al Mol Syst Biol. 2025 Dec 10. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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TetO::EGFP-hMeikin(8A mut)

Plasmid

#174707

Purpose

Doxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 binding

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Tags

EGFP

Expression

Mammalian

Mutation

S175A, T176A, T180A, S181A, S196A, T251A, T264A, …

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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AX44_pS.Donor.S1

Plasmid

#100289

Purpose

AAVS1-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

18 bp RFLP (MluI+KspAI)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pT076

Plasmid

#137880

Purpose

Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pT077

Plasmid

#137879

Purpose

Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS-HsProm1s1-StayGold

Plasmid

#211333

Purpose

Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Use

AAV

Tags

StayGold

Promoter

EF1a

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCLV3Cas-LT1

Plasmid

#210549

Purpose

Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM

Expression

Plant

Mutation

2bp insertion after AAVS1 target sequence that di…

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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LPUtopia-8

Plasmid

#224781

Purpose

Landing Pad donor vector for AAVS1 site integration in human and VeroE6 cells

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Inserts

puromycin N-acetyltransferase
enhanced Green Fluorescence Protein
Red Fluorescence Protein

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS-HsProm1s1(W795R)-StayGold

Plasmid

#211334

Purpose

Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (W795R)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Use

AAV

Tags

StayGold

Mutation

Changed Trp-795 to Arg

Promoter

EF1a

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCLV3Cas-LT2

Plasmid

#210550

Purpose

Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM

Expression

Plant

Mutation

1bp insertion after AAVS1 target sequence that di…

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-GAL9

Plasmid

#166689

Purpose

For creating a stable mCherry-GAL9 cell line

Depositor

Alan Sabirsh

Article

Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

Insert

LGALS9 (LGALS9 Human)

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

Plasmid

#126580

Purpose

A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Tags

HA

Expression

Mammalian

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2

Plasmid

#129715

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

no

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

Plasmid

#129668

Purpose

A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Tags

GFP

Expression

Mammalian

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA AAV 4a PhiC31 attP41

Plasmid

#242882

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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