We narrowed to 2,928 results for: BFP

1. pGL3-U6-sgRNA-BFP

   Plasmid

   #107722

   Purpose

   For expression of sgRNA from the U6 promoter with BFP expression simultaneously

   Depositor

   Xingxu Huang

   Article

   Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9::BFP-humanized

   Plasmid

   #44247

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 fused to BFP
   

   Use

   CRISPR and Retroviral

   Tags

   2xNLS & tagBFP

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1α-Cre-2A-mTagBFP2

   Plasmid

   #207746

   Purpose

   Expression of the Cre recombinase linked to a mTagBFP2 fluorescent marker

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   Cre-PGK-mTagBFP2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSCAR_sgRNA_blast-tagBFP-lox5171

   Plasmid

   #162072

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TagBFP2-FKBP-PIP5K1C_Kina-HD

   Plasmid

   #202741

   Purpose

   expresses recruitable fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mTagBFP2:FKBP1A(3-108):[GGSA]4GG:PIP5K1C(79-366)(D101R/R304D) (PIP5K1C Human)
   

   Expression

   Mammalian

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6-spTRE3G-CMV-mTagBFP2

   Plasmid

   #102854

   Purpose

   A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-TRE3G-sgRNA; mTagBFP2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mtagBFP2-CaM-uTEVp

   Plasmid

   #163033

   Purpose

   FLiCRE protease for neuronal expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   BFP-CaM-uTEVp
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TagBFP(P6f)3(P14f)3-gs

   Plasmid

   #228081

   Purpose

   Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

   Depositor

   Roman Jerala

   Article

   Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

   Insert

   BFP, P6f, P14f
   

   Expression

   Mammalian

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKH151_PB_flag-LC3B_wt_IRES-BFP

   Plasmid

   #214976

   Purpose

   Flag tagged LC3B

   Depositor

   Wade Harper

   Article

   Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

   Insert

   FLAG-LC3B (MAP1LC3B Human)
   

   Expression

   Mammalian

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUltra-H2B-mTagBFP2

    Plasmid

    #161791

    Purpose

    3rd generation lentiviral multicistronic vector for mammalian expression of EGFP, H2B-mTagBFP2, and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Multicistronic

    Tags

    EGFP and mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-EF1aL-TagBFP-W

    Plasmid

    #126687

    Purpose

    Constitutive, ubiquitous blue fluorescence protein (BFP) expression.

    Depositor

    Darrell Kotton

    Article

    Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

    Insert

    TagBFP
    

    Use

    Lentiviral

    Promoter

    Ef1aL

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRISPseq-BFP-backbone

    Plasmid

    #85707

    Purpose

    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

    Depositor

    Ido Amit

    Article

    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR Lck-TagBFP

    Plasmid

    #171034

    Purpose

    TagBFP-tagged Lck kinase

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

    Insert

    Lck
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-mTagBFP2-MRTF-A

    Plasmid

    #182061

    Purpose

    Mammalian expression vector encoding myocardin-related transcription factor A tagged with mTagBFP2.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mTagBFP2-MRTF-A (MRTFA Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKH152_PB_flag-LC3B_LDSmut_IRES-BFP

    Plasmid

    #214977

    Purpose

    Flag tagged LDS mutant LC3B

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    FLAG-LC3B_mut (MAP1LC3B Human)
    

    Expression

    Mammalian

    Mutation

    LDS mutant

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENO1-BFP-NAT

    Plasmid

    #50046

    Purpose

    Expression of BFP in Candida albicans

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

    Insert

    BFP
    

    Expression

    Yeast

    Promoter

    pENO1

    Available Since

    Jan. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCas9-P2A-BFP

    Plasmid

    #149447

    Purpose

    Expresses Cas9 and BFP in a lentiviral vector.

    Depositor

    Jonathan Chernoff

    Article

    Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

    Insert

    P2A-BFP
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCAR_sgRNA_blast-tagBFP-lox2272

    Plasmid

    #162071

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pR26 iBFP Dest

    Plasmid

    #74284

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2-H3.1-WT-BFP

    Plasmid

    #209437

    Purpose

    SB-transposable plasmid expressing FLAGGED human histone mutant H3.1-Wt with BFP

    Depositor

    Maria Castro

    Insert

    H3.1-WT
    

    Expression

    Mammalian

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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