We narrowed to 2,903 results for: BFP

1. CRISPseq-BFP-backbone

   Plasmid

   #85707

   Purpose

   Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

   Depositor

   Ido Amit

   Article

   Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-BFP-U6-sgRNA

   Plasmid

   #196725

   Purpose

   Sortable sgRNA expression, Chen tracrRNA PMID: 24360272

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCas9-P2A-BFP

   Plasmid

   #149447

   Purpose

   Expresses Cas9 and BFP in a lentiviral vector.

   Depositor

   Jonathan Chernoff

   Article

   Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

   Insert

   P2A-BFP
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   In frame with Cas9

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCAR_sgRNA_blast-tagBFP-lox2272

   Plasmid

   #162071

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR Src-TagBFP

   Plasmid

   #171036

   Purpose

   TagBFP-tagged Src kinase

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

   Insert

   Src
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mBFP-APPP1-mGFP

   Plasmid

   #196695

   Purpose

   As mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Tags

   mEGFP and mtagBFP2

   Expression

   Mammalian

   Mutation

   Lys to Val substitution at position 612

   Promoter

   CMV

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR26 iBFP Dest

   Plasmid

   #74284

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

   Plasmid

   #163169

   Purpose

   Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   Non-targeting
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAT9679-sgRNA-BFP

   Plasmid

   #162988

   Purpose

   sgRNA cloning plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

    Plasmid

    #155306

    Purpose

    Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Rfx Cas13d CRISPR RNA BFP
    

    Expression

    Mammalian

    Promoter

    hU6, EF1a

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ii-Str_ManII-SBP-tagBFP

    Plasmid

    #65257

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ii-Streptavidin and truncated Mannosidase II
    

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-TO-MCP-3XBFPnls

    Plasmid

    #75383

    Purpose

    MCP-3XBFPnls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    3XBFP

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLIX403-ccdB-EBFP2

    Plasmid

    #158562

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMIII-Neo-BFP

    Plasmid

    #153426

    Purpose

    Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    mTagBFP2
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. NES-mTagBFP2-P4Mx1

    Plasmid

    #108142

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):mTagBFP2:L. penomophila SidM(546-647)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. BFP Homology Donor

    Plasmid

    #149372

    Purpose

    Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Insert

    CDDR BFP Homology Donor
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-sgCDKN1B#2 BFP

    Plasmid

    #60905

    Purpose

    A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-mTagBFP2-ORP9-PH

    Plasmid

    #214267

    Purpose

    Mammalian expression of blue fluorescent PI4P reporter mTagBFP2-ORP9-PH

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    mTagBFP2-ORP9(1-111) (Osbpl9 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-h-mmPtch1-B-HA-TagBFP

    Plasmid

    #120910

    Purpose

    Expresses BFP tagged Ptch1-B

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. CROPseq-MPSV-eBFP2

    Plasmid

    #220472

    Purpose

    Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection

    Depositor

    Christian Krebs

    Article

    Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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