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pU6-BbsI-Torso

Plasmid

#244933

Purpose

CRISPR gRNA targeting the C-terminal end of Torso for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting Torso C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-Btl

Plasmid

#244934

Purpose

CRISPR gRNA targeting the C-terminal end of Breathless for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting Btl C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-EGFR

Plasmid

#244932

Purpose

CRISPR gRNA targeting the C-terminal end of EGFR for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting EGFR C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-NAT

Plasmid

#221129

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Expression

Yeast

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP24

Plasmid

#196762

Purpose

Contains MPK3 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

MITOGEN-ACTIVATED PROTEIN KINASE 3 (MPK3 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP26

Plasmid

#196764

Purpose

Contains MPK6 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

MITOGEN-ACTIVATED PROTEIN KINASE 6 (MPK6 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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NSUN6

Plasmid

#188060

Purpose

Bacterial expression plasmid for human NSUN6; for activity testing

Depositor

Tanja Schirmeister

Article

Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

Insert

NSUN6 (NSUN6 Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBV65

Plasmid

#108363

Purpose

A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

Depositor

Scott Moye-Rowley

Article

Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

Insert

loxP-natMX6-CgMET3prom-cre-loxP

Use

Gene disruption marker for candida glabrata

Promoter

C. glabrata MET3

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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NSUN2

Plasmid

#188059

Purpose

Bacterial expression plasmid for human NSUN2; for activity testing

Depositor

Tanja Schirmeister

Article

Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

Insert

NSUN2 (NSUN2 Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLC-mCherry-MLKL

Plasmid

#75167

Purpose

LentiCRISPR-mCherry with sgRNA targeting human MLKL

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

MLKL sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLC-EGFP-RIP3

Plasmid

#75165

Purpose

LentiCRISPR-EGFP with sgRNA targeting human RIP3

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

RIP3 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-CREB

Plasmid

#68550

Purpose

AAV expression of CREB

Depositor

Eric Nestler

Article

Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

Insert

CREB

Use

AAV

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLv-CMV-MyoD

Plasmid

#26808

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

MyoD (Myod1 Mouse)

Use

Lentiviral

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-mCREB

Plasmid

#68551

Purpose

AAV expression of mCREB

Depositor

Eric Nestler

Article

Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

Insert

mCREB

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

S133A

Promoter

CMV

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only
Cry2PHR.mCh.CofS3E

Plasmid

#133619

Purpose

Expresses Cry2PHR - mCherry - CofilinS3E protein fusion

Depositor

Robert Hughes

Article

Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

Insert

Cry2PHR - mCherry - CofilinS3E

Expression

Mammalian

Promoter

cmv

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-EGFP-RIP1

Plasmid

#75163

Purpose

LentiCRISPR-EGFP with sgRNA targeting human RIPk1

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

RIPK1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Actin.CIB.GFP

Plasmid

#133618

Purpose

Expresses beta actin - CIBN - GFP protein fusion

Depositor

Robert Hughes

Article

Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

Insert

betaActin - CIB

Tags

GFP

Expression

Mammalian

Promoter

cmv

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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ActinS14V.CIB.GFP

Plasmid

#133620

Purpose

Expresses beta actin S14V - CIBN - GFP protein fusion

Depositor

Robert Hughes

Article

Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

Insert

betaActin - CIB

Tags

GFP

Expression

Mammalian

Promoter

cmv

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHD-pYtag(Clover)-ScarlessDsRed

Plasmid

#244931

Purpose

Vector containing a Clover-based pYtag biosensor for CRISPR insertion into Drosophila RTKs, with a DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

Clover-tagged pYtag system (3xITAM P2A Clover-ZtSH2)

Use

CRISPR

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-pYtag(Scarlet)-ScarlessDsRed

Plasmid

#244938

Purpose

Vector containing a Scarlet-based pYtag biosensor for CRISPR insertion into Drosophila RTKs, with a DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

mScarlet-tagged pYtag system (3xITAM P2A mScarlet-ZtSH2)

Use

CRISPR

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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