We narrowed to 8,277 results for: gnal

1. pcDNA3.1_FLAG-YAP1

   Plasmid

   #173001

   Purpose

   constitutive expression of FLAG-tagged YAP1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   FLAG-YAP1 (YAP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1/Myc-His beta4

   Plasmid

   #16039

   Depositor

   Filippo Giancotti

   Article

   Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

   Insert

   beta4 (ITGB4 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV7.1_3xFLAG-LATS1

   Plasmid

   #172986

   Purpose

   constitutive expression of FLAG-tagged LATS1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-LATS1 (LATS1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCSIIpuro-mNG-eDHFR(69K6)-RasGEF

   Plasmid

   #172104

   Purpose

   Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti6_YAP1-V5

   Plasmid

   #173002

   Purpose

   constitutive expression of V5-tagged YAP1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   YAP1-V5 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV7.1_3xFLAG-WWC1

   Plasmid

   #172994

   Purpose

   constitutive expression of FLAG-tagged WWC1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-WWC1 (WWC1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pH6-TEV-spTorA-GFP(C)

   Plasmid

   #169041

   Purpose

   GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

   Depositor

   Siegfried Musser

   Article

   Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

   Insert

   H6-spTorA-GFP
   

   Use

   Tags

   6xHis, TEV cleavage site, and spTorA (signal pept…

   Expression

   Bacterial

   Mutation

   The mut3 GFP variant with 5 additional mutations …

   Promoter

   araBAD

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. His10-CD3z-SNAPf-ybbR (XSB507)

   Plasmid

   #78508

   Purpose

   Construct for expressing CD3z-SNAPf-ybbR in E. Coli

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   CD3z (Cytoplasmic domain) (CD247 Human)
   

   Use

   Tags

   His10 and SNAPf-ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB_Cdc42-TomKat-sensor-CAAX

   Plasmid

   #172473

   Purpose

   Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.

   Depositor

   Sean R Collins

   Article

   Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

   Insert

   tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
   

   Use

   Piggybac transposon

   Tags

   polybasic and CAAX motif from Kras, tdKatushka2, …

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV7.1_3xFLAG-STK3

    Plasmid

    #172990

    Purpose

    constitutive expression of FLAG-tagged STK3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-STK3 (STK3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcFLAG-CSK-delta SH3 (73 to450 amino acids)

    Plasmid

    #74504

    Purpose

    Overexpression of CSK delta SH3 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

    Insert

    CSK (CSK Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    del-SH3 (73 to450 amino acids)

    Promoter

    CMV

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

    Plasmid

    #62577

    Purpose

    Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. Flag‐mTim3 (T1 truncation)

    Plasmid

    #49210

    Purpose

    expresses Flag tagged mouse Tim3 (T1 truncation)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 T1 truncation (Havcr2 Mouse)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    T1 truncation ( contains all but the three most C…

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBridge-tyrosinase.σ3A

    Plasmid

    #197513

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-3 σ3A
    

    Use

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    silent substitution in codon 2 of tyrosinase tail…

    Promoter

    ADH1 and MET25

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP

    Plasmid

    #178857

    Purpose

    Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP (PRKCD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670

    Plasmid

    #178858

    Purpose

    Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)

    Plasmid

    #62579

    Purpose

    Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pMSCV-Flt3-ITD-Y591F/Y919F

    Plasmid

    #74499

    Purpose

    Retroviral expression of FLT3 ITD-Y591F/Y919F

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Y591F/Y919F, T227M

    Promoter

    unknown

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBridge-tyrosinase.σ2

    Plasmid

    #197415

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-2 σ2
    

    Use

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    encodes R42G substitution, contains silent substi…

    Promoter

    ADH1 and MET25

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

    Plasmid

    #178851

    Purpose

    Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Gαq: C9S/C10S/Q209L/L254A

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge