We narrowed to 4,504 results for: pcr

1. pCMV-TadA8e-PcrA M6

   Plasmid

   #221229

   Purpose

   Express HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-PcrA M6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-TagBFP-PuroR

   Plasmid

   #80969

   Purpose

   CRISPaint gene tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   TagBFP
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-3xP3-RFP

   Plasmid

   #130276

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   3xP3-RFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR-Flag-IKKbeta

   Plasmid

   #15465

   Depositor

   Hiroyasu Nakano

   Article

   Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

   Insert

   IkB kinase beta (Ikbkb Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pCR2.1-KRAS-G12V

   Plasmid

   #129091

   Purpose

   KI KRAS G12V mutation into human KRAS locus

   Depositor

   Toshiro Sato

   Article

   Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

   Insert

   KRAS(G12V) (KRAS Human)
   

   Use

   Homologous recombination donor plasmid

   Tags

   Expression

   Mutation

   G12V

   Promoter

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPaint-3xflag-PuroR

   Plasmid

   #80973

   Purpose

   CRISPaint gene tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   3xflag tag
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR Human CASP9 -2

   Plasmid

   #198420

   Purpose

   Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   Human CASP9 Specific gRNA (CASP9 Human)
   

   Use

   CRISPR

   Tags

   Cas9/Orange Fluorescent Protein Reporter

   Expression

   Mammalian

   Mutation

   Promoter

   U6; CMV

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR Human CASP9 -1

   Plasmid

   #198419

   Purpose

   Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   Human CASP9 Specific gRNA (CASP9 Human)
   

   Use

   CRISPR

   Tags

   Cas9/Orange Fluorescent Protein Reporter

   Expression

   Mammalian

   Mutation

   Promoter

   U6; CMV

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR8/GW/TOPO-ELK4

   Plasmid

   #98617

   Purpose

   Gateway entry TOPO TA cloning vector for ELK4

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK4 (Elk4 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC1825_pCR8-dCasRx-RBM38

    Plasmid

    #118662

    Purpose

    Gateway Donor; dCasRx-RBM38 ORF; No promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    dCasRx-RBM38
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

    Plasmid

    #89886

    Purpose

    Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-NRasQ61K
    

    Use

    Unspecified

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPR-cBEST-v2-PermE

    Plasmid

    #209446

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from ermE* promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC1821_pCR8-SMN2-DN-RG6-SA

    Plasmid

    #118655

    Purpose

    Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    SMN2-DN-RG6-SA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCri_L706-antiSH2-OptoMB

    Plasmid

    #159935

    Purpose

    Opto-MB bacterial expression

    Depositor

    Jared Toettcher, Jose Avalos

    Article

    Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

    Insert

    anti-SH2 MB with SS58 insert
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

    Plasmid

    #89887

    Purpose

    Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-HRasG12V
    

    Use

    Unspecified

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR8/GW/TOPO-ERF

    Plasmid

    #98626

    Purpose

    Gateway entry TOPO TA cloning vector ERF

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ERF (Erf Mouse)
    

    Use

    Topo ta cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCry2PHR-mCh-Rac1

    Plasmid

    #42954

    Purpose

    Expresses optogenetically controlled Rac1

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-Rac1 (RAC1 Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRT7 NT Topo arabinose

    Plasmid

    #73446

    Purpose

    Used for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptides

    Depositor

    Jesse Rinehart

    Type

    Empty backbone

    Use

    Tags

    GST (N-terminal), 6xHis (C-terminal)

    Expression

    Bacterial

    Mutation

    Promoter

    pBad

    Available since

    March 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC1816_pCR8-gSMN2-DN4

    Plasmid

    #118649

    Purpose

    Transient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-DN4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC149-pCR8-dCas9VP160

    Plasmid

    #48221

    Purpose

    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP160

    Expression

    Mutation

    D10A;H840A nuclease-deficient

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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