We narrowed to 4,553 results for: pcr

1. pCR3-FLAG-Gephyrin P1

   Plasmid

   #68816

   Purpose

   Flag tagged Geph mammalian expression

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   silent mutations on internal EcoRI site. Gephyrin…

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCRISPR-RFP entry

   Plasmid

   #192278

   Purpose

   Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   RFP flanked by type V-M CRISPR repeat sequences
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCry2PHR-mCh-RhoA

   Plasmid

   #42958

   Purpose

   Expresses optogenetically controlled RhoA

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPaint-3xP3-RFP

   Plasmid

   #130276

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   3xP3-RFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR-Flag-IKKbeta

   Plasmid

   #15465

   Depositor

   Hiroyasu Nakano

   Article

   Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

   Insert

   IkB kinase beta (Ikbkb Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pCRISPaint-3xP3-GFP

   Plasmid

   #130277

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   3xP3-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR2.1 GFR alpha 2

   Plasmid

   #119899

   Purpose

   Mouse GFR alpha 2 In situ hybridization probe

   Depositor

   David Ginty, Wenqin Luo

   Article

   Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

   Insert

   GDNF family receptor alpha-2 (Gfra2 Mouse)
   

   Use

   In situ hybridization probe

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPEY-GAL-Kan

   Plasmid

   #232099

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPEY-GAL-Hyg

   Plasmid

   #232098

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRII-BbsI-sgRNAscaffold

    Plasmid

    #159352

    Purpose

    Expression vector for a customizable guide RNA

    Depositor

    Pim Pijnappel

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR8/GW/TOPO BMI

    Plasmid

    #69796

    Purpose

    Gateway entry vector for BMI

    Depositor

    Gerard Cagney

    Insert

    BMI (BMI1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCR8/GW/TOPO PCGF2

    Plasmid

    #69791

    Purpose

    Gateway entry vector for PCGF2

    Depositor

    Gerard Cagney

    Insert

    PCGF2 (PCGF2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCRISPaint-3xflag-PuroR

    Plasmid

    #80973

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    3xflag tag
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRII-1.1-1.10a

    Plasmid

    #69589

    Purpose

    OT-II TCR a chain cDNA in pCRII

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Insert

    TCR alpha clone 1.1 (Tcra Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCR2.1-KRAS-G12V

    Plasmid

    #129091

    Purpose

    KI KRAS G12V mutation into human KRAS locus

    Depositor

    Toshiro Sato

    Article

    Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

    Insert

    KRAS(G12V) (KRAS Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR Human CASP9 -2

    Plasmid

    #198420

    Purpose

    Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

    Depositor

    Hannah Rabinowich

    Article

    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

    Insert

    Human CASP9 Specific gRNA (CASP9 Human)
    

    Use

    CRISPR

    Tags

    Cas9/Orange Fluorescent Protein Reporter

    Expression

    Mammalian

    Mutation

    Promoter

    U6; CMV

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPR Human CASP9 -1

    Plasmid

    #198419

    Purpose

    Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

    Depositor

    Hannah Rabinowich

    Article

    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

    Insert

    Human CASP9 Specific gRNA (CASP9 Human)
    

    Use

    CRISPR

    Tags

    Cas9/Orange Fluorescent Protein Reporter

    Expression

    Mammalian

    Mutation

    Promoter

    U6; CMV

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCry2PHR-mCh-Rac1

    Plasmid

    #42954

    Purpose

    Expresses optogenetically controlled Rac1

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-Rac1 (RAC1 Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC1825_pCR8-dCasRx-RBM38

    Plasmid

    #118662

    Purpose

    Gateway Donor; dCasRx-RBM38 ORF; No promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    dCasRx-RBM38
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC1821_pCR8-SMN2-DN-RG6-SA

    Plasmid

    #118655

    Purpose

    Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    SMN2-DN-RG6-SA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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