We narrowed to 9,400 results for: Coli

1. pET28a_LolT_C41S

   Plasmid

   #211789

   Purpose

   Expression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41S

   Depositor

   Yang Hai

   Article

   Gao et al Chembiochem. 2023 Oct 1:e202300561. doi:

   Insert

   lolt mutant
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBSV2_2

   Plasmid

   #118226

   Purpose

   Empty E. coli - B. burgdorferi shuttle vector; kanamycin resistant

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSB1C3-J23110-B0034-mRFP1_Magenta

   Plasmid

   #160446

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Magenta chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

   Insert

   promoter, RBS, mRFP1E_Magenta
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSB1C3-J23110-B0034-mRFP1_Yellow

   Plasmid

   #160443

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

   Insert

   promoter, RBS, mRFP1E_Yellow
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. vgCam-pRSETb

   Plasmid

   #193362

   Purpose

   A FRET-based calcium probe using Sumire

   Depositor

   Takeharu Nagai

   Article

   Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

   Insert

   vgCam
   

   Use

   E.coli expression

   Tags

   His X6 tag and Strep tag

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. A0A075HNX4

   Plasmid

   #177695

   Purpose

   expresses a fatty alcohol oxidase in E. coli

   Depositor

   Martin Engqvist

   Article

   Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

   Insert

   Long chain alcohol oxidase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEPQDKN0248

   Plasmid

   #162313

   Purpose

   Reporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   T7prom-RBS-sfGFP-HiBiT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBAD-hsGFP

   Plasmid

   #64911

   Purpose

   Encodes a Fluorescent Reporter for Hydrogen Sulfide in E. Coli

   Depositor

   Huiwang Ai

   Article

   Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74

   Insert

   hsGFP
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Chromophore tyrosine of the protein mutated to TA…

   Promoter

   araBAD

   Available Since

   May 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAllet

   Plasmid

   #202560

   Purpose

   E. coli cloning vector with large MCS (50+ unique restriction sites)

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pG1AK-mCherry

    Plasmid

    #71737

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Insert

    mCherry
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRplsWT

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3.1_CoumRS

    Plasmid

    #64882

    Purpose

    Expresses Coumarinyl- tRNA synthetase

    Depositor

    Sebastián Brauchi

    Article

    Steinberg et al bioRxiv, 10.1101/065227

    Insert

    Coum_RS
    

    Use

    Tags

    Flag

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-ChmN

    Plasmid

    #221348

    Purpose

    Express ChmN in E. coli

    Depositor

    Emily Balskus

    Article

    Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

    Insert

    chmN
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSB1C3-J23110-B0034-mRFP1_Pink

    Plasmid

    #160445

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Pink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1E_Pink
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKL13-MgtC bb118

    Plasmid

    #158982

    Purpose

    Constitutive expression (sigma 70 promoter) of mgtC protein from S. Typhimurium in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    mgtC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_J23118 (sigma 70)

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid

    #208636

    Purpose

    for E. coli expression of human eIF5B (ENST00000289371)

    Depositor

    Quanfu Ma

    Insert

    human eIF5B (ENST00000289371) (EIF5B Human)
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Contain SNP rs1376443, protein sequence not chang…

    Promoter

    T7

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSB1C3-J23110-B0034-mRFP1_Orange

    Plasmid

    #160444

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1E_Orange
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCherryS131C

    Plasmid

    #48732

    Purpose

    Expresses mCherryS131C in E. coli cells

    Depositor

    Bradley Olsen

    Article

    Thomas et al ACS Nano. 2011 Jul 26;5(7):5697-707. doi

    Insert

    mCherryS131C
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed serine 131 to cysteine

    Promoter

    T5

    Available Since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMAL-c5X-35-H-6

    Plasmid

    #67008

    Purpose

    Expresses malE-35-H-6 in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-35-H-6
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPQD0KN0245

    Plasmid

    #162284

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPQDKN0729

    Plasmid

    #162316

    Purpose

    TEV protease plasmid for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    T7prom-RBS-NET-TEVprotease
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-SznE

    Plasmid

    #139282

    Purpose

    expresses SznE in E. coli

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    sznE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMOD4 galK-GT

    Plasmid

    #19183

    Depositor

    Al Fisher

    Article

    Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

    Insert

    galK (galK E.coli)
    

    Use

    Recombineering

    Tags

    50 nucleotides from N-term TAP, C-term TAP, and F…

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-PAN

    Plasmid

    #200971

    Purpose

    Express poly-histidine tagged Methanococcus jannaschii PAN in E. coli recombinantly

    Depositor

    Yifan Cheng

    Article

    Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

    Insert

    PAN
    

    Use

    Tags

    6xHis and 6xHis-thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-hdNadV

    Plasmid

    #83362

    Purpose

    Expresses nadV from Haemophilus ducreyi in Escherichia coli

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

    Insert

    nadV
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBbS5c-AcrB/AcrD/AcrFa, ABO_0964

    Plasmid

    #86206

    Purpose

    Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

    Depositor

    Mary Dunlop

    Article

    Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

    Insert

    Efflux pump
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pND004

    Plasmid

    #200950

    Purpose

    MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

    Depositor

    Timothy Whitehead

    Article

    Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEDL3

    Plasmid

    #25092

    Depositor

    Christopher Voigt

    Article

    Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

    Insert

    Edge detector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAW-SpyCsn2

    Plasmid

    #113251

    Purpose

    E. coli expression vector for S. pyogenes Csn2-MBP-His

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Csn2
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSETb-pHlash

    Plasmid

    #61550

    Purpose

    Fusion of Rluc8 and cpVenus. Expression of pHlash in Escherichia coli for protein purification

    Depositor

    Carl Johnson

    Article

    Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

    Insert

    pHlash
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pAW-SpyCas1

    Plasmid

    #113248

    Purpose

    E. coli expression vector for His-MBP-S. pyogenes Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Cas1
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge