We narrowed to 3,810 results for: luciferase expression
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Plasmid#168127PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F125/D126 within GNA13PromoterAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only
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Go1-CASE
Plasmid#168123PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at L91/G92 within GNAO1PromoterAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR T757
Plasmid#140828PurposeAIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cellsDepositorInsertAIMTOR T757 (ULK1 Human)
UseTagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of humā¦PromoterAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
2XSmBiT-SARS-CoV-2 S1
Plasmid#173430Purposeprotein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 FcDepositorInsert2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)
UseLuciferaseTagshuman IgG1 FcExpressionMammalianMutationfurin cleavage site RRAR to GSASPromoterCMVAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0043-PspCas13b-gRNA non target
Plasmid#223698PurposegRNA control for dPspCas13b-FTODepositorInsertgRNA target sequence for luciferase control
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEXSISERS_BSD_FRT-PuroR
Plasmid#177867PurposeCloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassetteDepositorInsertBSD-based EXSISERS
UseCRISPR, Luciferase, Synthetic Biology, and Unspecā¦TagsOLLASExpressionMammalianMutationPromoterAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiR-report-MYB-WT
Plasmid#141114PurposeReporter plasmid of WT MYB in mammalian cells.DepositorAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmiR-report-MYB-mut
Plasmid#141115PurposeReporter plasmid of mutant MYB in mammalian cells.DepositorInsertMYB (MYB Human)
UseLuciferaseTagsExpressionMammalianMutationA to T mutations at m6A sitesPromoterAvailable SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Luc.Cre empty vector
Plasmid#20905DepositorTypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable SinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pNH-NanoLuc
Plasmid#173075PurposeExpression vector for E. coli producing target protein with N-terminal Nanoluciferase fusionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-FTO promoter_WT
Plasmid#108922PurposeThe core promoter region of FTO was inserted into pGL3 basic vectorDepositorAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-FTO promoter_Mut
Plasmid#108923PurposeThe core promoter region of FTO with mutant CEBPA binding sites was inserted into pGL3 basic vectorDepositorAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIR-REPORT-MYC CDS_WT
Plasmid#108924PurposeMYC mRNA CDS region with wild type m6A motifs was inserted into pMIR-REPORT vectorDepositorAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ubc.IkBSR-Flag Pgk.Cre
Plasmid#22503DepositorInsertIkBSR
UseCre/Lox and LentiviralTagsFlagExpressionMammalianMutationPromoterAvailable SinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mU6-Luc2GO-PGK-Neo
Plasmid#136905PurposeLentivirus for base editing activatable Luciferase2 expression in mammalian cells. All in one vector with sgLuc2GO.DepositorInsertLuc2CyGo
UseLentiviralTagsExpressionMutationATG>ACGPromoterSFFVAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
L2092-GPA-FullBRET-Retrovirus-TD142
Plasmid#222877PurposeRetroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.DepositorInsertFRB-GPA-NanoLuc-CyOFP1
UseRetroviralTagsMycExpressionMammalianMutationPromoterMSCV LTRAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2125-FRB-GPA-NLuc-CyOFP1
Plasmid#222885PurposePositive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.DepositorInsertFRB-GPA-20GS-NanoLuc-10GS-CyOFP1
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2096-GPA-BRET-Biosensor-Retrovirus-TD146
Plasmid#222878PurposeRetroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.DepositorInsertFRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
UseRetroviralTagsMycExpressionMammalianMutationPromoterMSCV LTRAvailable SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only