We narrowed to 9,183 results for: try;

1. pLuc-OptoJNKi

   Plasmid

   #89744

   Purpose

   Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-2B-Nkx2-5YRD Y-A

   Plasmid

   #98611

   Purpose

   Gateway entry vector for NKX2-5 YRD Y-A

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 YRD Y-A (Nkx2-5 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Substitutions of 7 tyr into Ala residues.Y233A; Y…

   Promoter

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. KC1G3_HUMAN_D0

   Plasmid

   #79685

   Purpose

   This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KC1G3 (CSNK1G3 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. csd6_13-330_W158A/pET28b(+) N-tag

   Plasmid

   #71358

   Purpose

   csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 changed Tryptophan 158 to Alanine)
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pFOSGE_COF4D03

   Plasmid

   #70969

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Taf12 (Taf12 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pFOSGE_CUS1F03

   Plasmid

   #71071

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Su(var)205 (Su(var)205 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pDONR221-ARL4D-no stop

   Plasmid

   #67337

   Purpose

   hArl4D without stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4D (ARL4D Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Bacterial

   Mutation

   bp 272 A to C; N91T mutation; bp 327 T to G; sil…

   Promoter

   None

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pDONR221-ARF4-no stop

   Plasmid

   #67311

   Purpose

   hArf4 without stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF4 (ARF4 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Bacterial

   Mutation

   V53A, L123P and M134I mutations were unintended P…

   Promoter

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pENTR1A DHFR.dn-cHSF1

   Plasmid

   #134726

   Purpose

   Gateway entry vector for constitutively active dominant-negative HSF1 fused to the DHFR destabilized domain

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   DHFR.dn-cHSF1 (HSF1 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   DHFR

   Expression

   Mutation

   Promoter

   None

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKK-TEV-EGFP

    Plasmid

    #105793

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKK-BiFC-Venus

    Plasmid

    #105804

    Purpose

    Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    VN173 (I152L); VC155

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-neo:Beta2meGFP

    Plasmid

    #45096

    Depositor

    Henry Lester

    Article

    Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

    Insert

    nAChR beta2 (Chrnb2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    There are strain differences between C57BJ/6 and …

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E ubb polyA

    Plasmid

    #195977

    Purpose

    Gateway p3E vector

    Depositor

    Christian Mosimann, Alexa Burger

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubb:polyA (ubb Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nEF-Coff/Fon DREADD Gi-mCherry

    Plasmid

    #177669

    Purpose

    Expresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cells

    Depositor

    Karl Deisseroth

    Article

    Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC1-V16-eGFP

    Plasmid

    #122033

    Purpose

    AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and eGFP expression

    Depositor

    Liliane Tenenbaum

    Article

    Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT3EF5a-PIK3CA H1047R

    Plasmid

    #180034

    Purpose

    Entry vector of PIK3CA H1047R for gateway cloning.

    Depositor

    Xin Chen

    Article

    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Entry vector

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. DYR1A_HUMAN_D0

    Plasmid

    #79690

    Purpose

    This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    DYRK1A (DYRK1A Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. p5E-Runx1+23

    Plasmid

    #69602

    Purpose

    Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning

    Depositor

    Owen Tamplin, Leonard Zon

    Article

    Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:

    Inserts

    Runx1 +23 intronic enhancer (Runx1 Mouse)
    Mouse beta-globin minimal promoter
    

    Use

    5' gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    C57/BL6 mouse Runx1 +23 intronic enhancer and C57…

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKK-BI16-TEV-mCherry

    Plasmid

    #105800

    Purpose

    Concomitant expression of two proteins. One protein is expressed with mCherry fusion tag (at the C-terminus), cleavable by TEV protease; second tag introduced by user.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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