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YSBE 557 : pNAT_GFP

Plasmid

#92085

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, NAT selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

GFP

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRosea (GB0026)

Plasmid

#68194

Purpose

Provides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

A. majus Rosea coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-mir-92

Plasmid

#64092

Purpose

expresses miR-92

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

miR-92 (MIR92A1 Human)

Expression

Mammalian

Promoter

MSCV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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shBCL2L1 # 1

Plasmid

#42551

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shBCL2L1 (BCL2L1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
shBCL2L1 # 2

Plasmid

#42552

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shBCL2L1 (BCL2L1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+MT-hToca-1-W518K

Plasmid

#33031

Depositor

Marc Kirschner

Article

Ho et al Cell. 2004 Jul 23;118(2):203-16.

Insert

Toca-1 (FNBP1L Human)

Tags

6xMyc

Expression

Mammalian

Mutation

W518K

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
HA Raptor mutant 3

Plasmid

#8473

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

313NWIF316-AAAA (N313A, W314A, I315A, F316A)

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pCMV7.1_3xFLAG-LATS1

Plasmid

#172986

Purpose

constitutive expression of FLAG-tagged LATS1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-LATS1 (LATS1 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLGR002 LGR Cas9 guide vector

Plasmid

#188320

Purpose

Universal backbone for CRISPR/Cas9 sgRNA expression

Depositor

Laralynne Przybyla

Article

Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

PuroR-T2A-mTagBFP2

Expression

Mammalian

Promoter

pU6 5'CMV and EF1-alpha

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pFlox_N-p300_BSD_FKBPF36V_EYFP

Plasmid

#163885

Purpose

Acute Depletion P300-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

P300 (Ep300 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pHis-hPim1

Plasmid

#100722

Purpose

Expression of human Pim1 kinase domain in E.coli

Depositor

Byung Il Lee

Article

Lee et al PLoS One. 2013 Jul 31;8(7):e70358. doi:

Insert

Pim1 (PIM1 Human)

Tags

His

Expression

Bacterial

Mutation

Residues 29-313 of isoform 2

Promoter

T7 promotor

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-YAP1 CA

Plasmid

#79502

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

YAP1 (YAP1 Human)

Use

Gateway Cloning

Tags

3XFLAG

Mutation

S127A

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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MO70-mJP-4

Plasmid

#79598

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

Insert

Junctophilin-4 (Jph4 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pHygromycin (GB0211)

Plasmid

#68204

Purpose

Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hygromycin resistance gene coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCool-hDDB1-BPB

Plasmid

#48639

Purpose

Expresses DDB1 BPB domain (aa 391-709) in Ecoli (ampR)

Depositor

Ning Zheng

Article

Li et al Cell. 2006 Jan 13;124(1):105-17.

Insert

hDDB1-BPB (DDB1 Human)

Expression

Bacterial

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only
pAO139 (Apr LEU2 mrcl-AQ)

Plasmid

#41924

Depositor

Stephen Elledge

Article

Osborn et al Genes Dev. 2003 Jul 15;17(14):1755-67.

Insert

Mediator of the Replication Checkpoint

Mutation

MRC1AQ

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-COPE

Plasmid

#31585

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

coatomer protein complex, subunit epsilon (COPE Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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psiCHECK SCN1B 3'UTR

Plasmid

#19855

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

SCN1B 3'UTR (SCN1B Human)

Expression

Mammalian

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag PA1

Plasmid

#15560

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PA1 (PAGR1 Human)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
PT7lacO-His6-AlaRS

Plasmid

#224362

Purpose

Express AlaRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

AlaRS (alaS E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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