We narrowed to 23,118 results for: Sis
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Plasmid#153439PurposeE. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc.XDocB
Tags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b K294R
Plasmid#118359PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S307A
Plasmid#118364PurposeThis plasmid expresses human HSF1 S307A mutant.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1498
Plasmid#82427PurposePlasmid for expression of NHA-tagged Nematostella vectensis GW182 11W11A in mammalian cellsDepositorInsertnvGW182 11W11A
TagsNHAExpressionMammalianMutationTrp 1167, 1183, 1227, 1309, 1391, 1453, 1574, 158…PromoterCMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a S620T
Plasmid#53059PurposeExpresses alpha subunit of S620T hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionMutationchanged Serine 620 to ThreoninePromoterSP6Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-zeo
Plasmid#160091PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance markerDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_POU2F3_CR
Plasmid#122175PurposeLentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertPOU2F3 (POU2F3 Human)
UseLentiviralExpressionMammalianMutation3 silent mutations for gRNA resistance (882g-c, 8…PromoterEFSAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIDTSmart-CMV-PLRS1
Plasmid#217365PurposeExpression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"DepositorInsertleucyl-tRNA synthetase polyspecific mutant
ExpressionMammalianMutationM40I, T252A, Y499I, Y527A, H529GPromoterCMVAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pICE-RNaseHI-WT-NLS-mCherry
Plasmid#60365PurposePlasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI
TagsNLS from SV40 T antigen and mCherryExpressionMammalianPromoterCMV-tetAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HA-L2HGDH(opt)-FLAG
Plasmid#230089Purposeexpressing human L2HGDHDepositorInsertL2HGDH (L2HGDH Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationMultiple wobble mutationsPromoterCMVAvailable SinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-HA-NEK7
Plasmid#75142PurposeExpresses N-terminal HA-tagged NEK7 in mammalian cellsDepositorInsertNIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
TagsHAExpressionMammalianPromoterCMVAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1_puro_shNRF2-1
Plasmid#230086PurposeshRNA silencing human NRF2 geneDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseLentiviral and RNAiExpressionMammalianAvailable SinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgFSP1
Plasmid#186026Purposeknock out FSP1 in mammalian cellsDepositorAvailable SinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBMNZ-neo-Flag-TNFR1 L380A
Plasmid#43949DepositorAvailable SinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-KATNA1
Plasmid#180650PurposeLentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.DepositorInsertKATNA1 (KATNA1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GGACAGCACUCCCUUGAAAAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-hygro
Plasmid#160090PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance markerDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB2D
Plasmid#195286PurposeBicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selectionDepositorInsertDTR EGFP 2A Puro
ExpressionMammalianPromoterEFSAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB2E
Plasmid#195288PurposeBicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selectionDepositorInsertHSV-TK EGFP 2A Puro
ExpressionMammalianPromoterEFSAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICE-FLAG-NAT10-siR-WT
Plasmid#59365PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInserthuman NAT10 (NAT10 Human)
TagsFLAGExpressionMammalianMutationMutations to render cDNA resistant to a siRNAPromoterCMV-tetAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1_puro_shNRF2-2
Plasmid#230087PurposeshRNA silencing human NRF2 geneDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseLentiviral and RNAiExpressionMammalianAvailable SinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only