We narrowed to 13,583 results for: TIM

1. pCRISPR-S12

   Plasmid

   #84031

   Purpose

   To episomally express codon optimized Cas9 and chimeric guide RNA

   Depositor

   Sen Wu

   Article

   Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

   Inserts

   hSpCas9
   eGFP
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and CMV (downstream of F2A self-cleaving pept…

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. vYM112_PB-pEF1a-optiPIN2-HA(Hygro)

   Plasmid

   #160045

   Purpose

   For expressing the active auxin pump PIN2

   Depositor

   Michael Elowitz

   Article

   Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

   Insert

   PIN2
   

   Use

   Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   pEF1s

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYZ959

   Plasmid

   #192603

   Purpose

   TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   ScTEF1

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYZ960

   Plasmid

   #187972

   Purpose

   TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIntegrase_2

   Plasmid

   #60574

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMV261-Cas12a

   Plasmid

   #158707

   Purpose

   expresses codon-optimized FnCpf1

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   FnCpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOSML-Cox2-GFP

   Plasmid

   #203711

   Purpose

   Overexpression of COX2-GFP fusion

   Depositor

   Timothy Hla

   Article

   Trifan et al J Biol Chem. 1999 Nov 26;274(48):34141-7

   Insert

   Cox2-GFP (PTGS2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SV40

   Available since

   June 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PP244

   Plasmid

   #78978

   Purpose

   Promoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSET-dCas9-actII-4-NT-S1

   Plasmid

   #110185

   Purpose

   Repression of gene expression in Streptomyces by CRISPRi

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Inserts

   dCas9
   sgRNA
   

   Use

   CRISPR; I

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   J23119 and ermE*p

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Gi-c010

    Plasmid

    #173080

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GAP
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    mCitrine + last 10 residues of GNA1, codon optimi…

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDIV296

    Plasmid

    #204687

    Purpose

    AMA1 plasmid with nat1 resistance marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Insert

    nat1
    

    Use

    Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC57-PP7v3

    Plasmid

    #107230

    Purpose

    Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    24xPP7v3
    

    Use

    Tags

    3x FLAG-tag and P2A site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSBY1_FnCpf1cg

    Plasmid

    #104622

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    codon-optimized C.glutamicum

    Promoter

    hsp60

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2+_NLS-Tir1-HA

    Plasmid

    #117716

    Purpose

    mRNA synthesis of NLS-TIR1-HA for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    TRANSPORT INHIBITOR RESPONSE 1
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    codon-optimized TIR1 for murine expression

    Promoter

    SP6

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA6 MCV sTco

    Plasmid

    #40201

    Depositor

    Patrick Moore

    Article

    Shuda et al J Clin Invest. 2011 Sep;121(9):3623-34.

    Insert

    MCPyV sT codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBLO1811_Cas9_noNLS_human

    Plasmid

    #74490

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DdMCU

    Plasmid

    #67642

    Purpose

    DdMCU-Flag in pACT2 vector human codon optimised

    Depositor

    Vamsi Mootha

    Article

    Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    DdMCU
    

    Use

    Tags

    Flag tag with linker

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPP394

    Plasmid

    #158800

    Purpose

    Plasmid containing Homo sapiens codon optimized CasΦ-1 and a SapI-GG stuffer spacer for editing in human cells.

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBLO1808_arC9_2xNLS_human

    Plasmid

    #74491

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBLO1806_Cas9_2xNLS_human

    Plasmid

    #74493

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMSSM16: pCMV-HA-NLS-SpuFz1

    Plasmid

    #205271

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Human p150,95

    Plasmid

    #8633

    Depositor

    Timothy Springer

    Article

    Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

    Insert

    Human p150,95 (ITGAX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mt TEFM

    Plasmid

    #205058

    Purpose

    TEFM protein expression

    Depositor

    Smita Patel

    Article

    Sultana et al J Biol Chem. 2017 Nov 3;292(44):18145-18

    Insert

    TEFM (TEFM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. lentiCRISPR - AAVS1 sgRNA

    Plasmid

    #70661

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against AAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pDD121

    Plasmid

    #91833

    Purpose

    Cas9 expression in C. elegans

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p4RTK GL4.10 (Luc2)

    Plasmid

    #16057

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Multimerized E-box
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

27. pSBY3_ligD

    Plasmid

    #104624

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIntegrase_7

    Plasmid

    #60578

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLN592 (FuGW-S(USF1)p-mKate2)

    Plasmid

    #105204

    Purpose

    synthetic promoter USF1 drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(USF1)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLN382 (FuGW-S(HIF1A)p-mKate2 )

    Plasmid

    #105180

    Purpose

    synthetic promoter HIF1A drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(HIF1A)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pfMDH_WT

    Plasmid

    #67721

    Purpose

    Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pZ8-P_dCas9

    Plasmid

    #74063

    Purpose

    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    dcas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Propionate inducible promoter (prp)

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pIREShygro HA-mVenus-mAID

    Plasmid

    #117714

    Purpose

    Expression of HA-tagged mVenus fused to mAID

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    mVenus-mAID
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMYO127TAG-SepT

    Plasmid

    #34625

    Depositor

    Dieter Söll, Jesse Rinehart

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    Myoglobin-127TAG
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    codon-optimized + an amber stop codon was introdu…

    Promoter

    Ipp

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMLS485

    Plasmid

    #188890

    Purpose

    Worm Cas9 expression vector, Peft-3 promoter

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Peft-3::Cas9
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA-DEST53-mTrific

    Plasmid

    #51031

    Purpose

    mammalian expression of mouse Trific fused to GFP

    Depositor

    Timothy Cox

    Article

    Short et al J Biol Chem. 2006 Mar 31;281(13):8970-80

    Insert

    Trific (Trim46 Mouse)
    

    Use

    Tags

    Cycle3 GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMSP2

    Plasmid

    #26282

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009 . 464():211-31.

    Insert

    MSP2
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of dimer of deletion mutant (1-43)…

    Promoter

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

38. Integrin-β6 Headpiece I270C

    Plasmid

    #159638

    Purpose

    Contains integrin beta 6 residues 1–474 with I270C mutation

    Depositor

    Timothy Springer

    Article

    Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

    Insert

    Integrin beta 6 headpiece I270C
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    I270C

    Promoter

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pIntegrase_5

    Plasmid

    #60577

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMH220

    Plasmid

    #122836

    Purpose

    dCas9 with GreenGate B-C flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Contains D10A and H840A mutations

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEG476

    Plasmid

    #40198

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (S404A) (mex-5 Nematode)
    

    Use

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Expression

    Worm

    Mutation

    S404A

    Promoter

    pie-1

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

42. pSpCas9n(BB)-2A-puro-a5 guide B

    Plasmid

    #194011

    Purpose

    For CRISPR knockout of integrin alpha5

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (a5_guide B)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSpCas9n(BB)-2A-puro-aV guide A

    Plasmid

    #194008

    Purpose

    For CRISPR knockout of integrin alphaV

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (aV_guide A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSpCas9n(BB)-2A-puro-aV guide B

    Plasmid

    #194009

    Purpose

    For CRISPR knockout of integrin alphaV

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (aV_guide B)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSpCas9n(BB)-2A-puro-a5 guide A

    Plasmid

    #194010

    Purpose

    For CRISPR knockout of integrin alpha5

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (a5_guide A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pEBG-NHERF2

    Plasmid

    #28292

    Depositor

    Maria-Magdalena Georgescu

    Article

    Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

    Insert

    NHERF2 (NHERF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

47. pNR291

    Plasmid

    #79555

    Purpose

    Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Inserts

    GFPmut3b
    mTagBFP
    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCAG-T3-eSpCAS9-NG-pA

    Plasmid

    #131466

    Purpose

    Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

    Insert

    Codon optimized eSpCas9-NG
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCRISPomyces-Sth1Cas9

    Plasmid

    #129552

    Purpose

    Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

    Depositor

    Mingzi M. Zhang, Fong Tian Wong

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    Sth1Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pGGB-SpCas9

    Plasmid

    #210533

    Purpose

    Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    SpCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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