We narrowed to 12,322 results for: BASE

1. p663-UBC-miniTurbo-V5-DCLK3_IDG-K

   Plasmid

   #189891

   Purpose

   Gateway destination clone of DCLK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   DCLK3 (DCLK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p667-UBC-ADCK2-V5-miniTurbo_IDG-K

   Plasmid

   #189908

   Purpose

   Gateway destination clone of ADCK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   ADCK2 (ADCK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPpT4- HTA1prom-TIR1-FLAG

   Plasmid

   #189725

   Purpose

   Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   HTA1

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPpT4- TEF2prom-TIR1-FLAG

   Plasmid

   #189726

   Purpose

   Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   TEF2

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pM159

   Plasmid

   #173215

   Purpose

   Expresses dCas9-sfGFP10

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   dCas9-sfGFP10M2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2370-Tier1-PhCMV-TetR_F-type

   Plasmid

   #169573

   Purpose

   Tier-1 vector encoding PhCMV-driven tetracycline-dependent F-type-based transactiavtor (PhCMV-TetR-F-type-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled transactivator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CSM160

   Plasmid

   #105684

   Purpose

   Plasmid containing an RFP dropout used to construct randomized PAM library

   Depositor

   Chase Beisel

   Article

   Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

   Insert

   RFP Golden Gate dropout
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_R0040 TetR Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMCL8

   Plasmid

   #176184

   Purpose

   gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMCL8_28bp

   Plasmid

   #176185

   Purpose

   plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMCL9

    Plasmid

    #176186

    Purpose

    2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pColE1-AlphaOmega

    Plasmid

    #165867

    Purpose

    Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBlastR-GMRWhite

    Plasmid

    #165885

    Purpose

    Blasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165888

    Purpose

    Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165889

    Purpose

    Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGal4BD 1-147

    Plasmid

    #165819

    Purpose

    1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Gal4BD 1-147
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGAL4AD 768-881

    Plasmid

    #165820

    Purpose

    768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    GAL4AD 768-881
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p663-UBC-miniTurbo-V5-LMTK2_IDG-K

    Plasmid

    #170580

    Purpose

    Gateway destination clone of LMTK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-LMTK2 (LMTK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pM80

    Plasmid

    #173173

    Purpose

    HA-dCas9

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p663-UBC-miniTurbo-V5-EEF2K_IDG-K

    Plasmid

    #170592

    Purpose

    Gateway destination clone of EEF2K (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-EEF2K (EEF2K Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-TK-Firefly-cjCD69

    Plasmid

    #122279

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjCD69 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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