We narrowed to 5,421 results for: PEP

1. pNK869

   Plasmid

   #219737

   Purpose

   MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

   Insert

   promoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtPD7

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP

   Plasmid

   #153008

   Purpose

   Expresses glucagon peptide on mammalian cell surface

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   Glucagon-ICAM1-pre-mGRASP
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOCC154

   Plasmid

   #118902

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Use

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV_Lyn11-mTurquoise2-iLID

   Plasmid

   #161000

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   Lyn11-mTurquoise2-iLID
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNAS1B split mCherry N-link/link-C

   Plasmid

   #61965

   Purpose

   Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

   Depositor

   Lynne Regan

   Article

   Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

   Inserts

   N-mCherry-link
   link-C-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD and PLtetO

   Available since

   Feb. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-V96 ELP

   Plasmid

   #67853

   Purpose

   GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

   Depositor

   Andrew MacKay

   Article

   Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

   Insert

   GFP fused to ELP (VPGVG) repeated 96 times
   

   Use

   Tags

   GFP fused to N-terminal of ELP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOCC153

   Plasmid

   #118901

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4TO-Kif18b-24xGCN4_v1

   Plasmid

   #72228

   Purpose

   A fusion of the Kif18b motor protein to the v1 SunTag peptide array

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   Kif18b (KIF18B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Tras light chain

   Plasmid

   #216294

   Purpose

   Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Pert Light chain

    Plasmid

    #216310

    Purpose

    Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Pert Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. MX01

    Plasmid

    #158624

    Purpose

    GATEWAY and BacMam compatible mammalian expression vector with eGFP expression reporter (eGFP is expressed by same promotor but as a separate polypeptide chain).

    Depositor

    Timm Maier

    Article

    Scaiola et al Sci Adv. 2020 Nov 6;6(45). pii: 6/45/eab

    Type

    Empty backbone

    Use

    Gateway destination vector ; bacmam acceptor vect…

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid

    #61966

    Purpose

    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD and PLtetO

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)

    Plasmid

    #192123

    Purpose

    Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pStr-KDEL_APV-SBP-DsRed2-ER-5

    Plasmid

    #170675

    Purpose

    Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Inserts

    APV-SBP-DsRed2-ER-5
    streptavidin-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and T7

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Clathrin-LCa-ECFP

    Plasmid

    #21742

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pSCM167.2-Venus-VF-hADAM10(aa1-213)

    Plasmid

    #189554

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 1-213

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. hPDI1-18-508_C400S_C56S-pFLAG-CMV1

    Plasmid

    #31383

    Depositor

    David Ron

    Article

    Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

    Insert

    PDI1A (P4HB Human)
    

    Use

    Tags

    FLAG M2

    Expression

    Mammalian

    Mutation

    C-terminal cysteines of both active sites mutated…

    Promoter

    Available since

    May 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLSU4cg10

    Plasmid

    #126078

    Purpose

    Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    longCaMV35S

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p4459 FLAG-hPLIC-1 NTF

    Plasmid

    #8664

    Depositor

    Peter Howley

    Article

    Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

    Insert

    hPLIC-1 (UBQLN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N-terminal fragment: amino acids 1-112 of hPLIC-1…

    Promoter

    Available since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pDEST14-SpyCatcher002 EQ

    Plasmid

    #102830

    Purpose

    Bacterial expression of unreactive version of SpyCatcher002

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyCatcher002 EQ
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    E77Q mutation prevents isopeptide bond formation

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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