We narrowed to 5,882 results for: arl

1. 5` CC: Puro – loxP – GFP-C

   Plasmid

   #219563

   Purpose

   5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   hPGK-promoter_PuroR_2A_loxP_GFP-C
   

   Use

   Unspecified

   Promoter

   hPGK

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti6/V5-DEST-RNF2

   Plasmid

   #31216

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   RNF2 (RNF2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   April 26, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pGEX6P1-3xMBT

   Plasmid

   #46987

   Purpose

   Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli

   Depositor

   Or Gozani

   Article

   Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

   Insert

   Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
   

   Tags

   GST and PreScission cleavage site

   Expression

   Bacterial

   Mutation

   contains amino acids 190–530 of accession NP_0562…

   Promoter

   tac

   Available Since

   Sept. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-Cas9-T2A-TdT

   Plasmid

   #126424

   Purpose

   Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-T2A-TdT (DNTT Streptococcus pyogenes, Human)
   

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

   Plasmid

   #84243

   Purpose

   Expresses ABA-inducible VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sa dCas9
   PYL1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

   Expression

   Mammalian

   Mutation

   D10A, N580A and Synonymous mutation in PYL1 to re…

   Promoter

   CAG and PGK

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSSRB070_pAC8-hsCRBN

   Plasmid

   #218789

   Purpose

   Insect cell expression vector for FLAG-TEV-Spy-hsCRBN

   Depositor

   Eric Fischer

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   Protein cereblon (CRBN Human)
   

   Tags

   Flag-TEV-Spy

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti-AcGFP-Sec61-IRES-Blast

   Plasmid

   #154009

   Purpose

   Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   acGFP-Sec61 (SEC61B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR221-TMEM165

   Plasmid

   #132272

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   TMEM165 (TMEM165 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pSD025A

   Plasmid

   #218128

   Purpose

   Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of gIII-neg

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Inserts

   CRBN (CRBN Human)
   M13 pIII-neg
   

   Tags

   scP22

   Mutation

   W384A, Y386A

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tet-O-FUW-TRPS1-P2A-mCherry

    Plasmid

    #203851

    Purpose

    Tet-inducible lentiviral plasmid expressing TRPS1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    TRPS1 (TRPS1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTwist-SARS-CoV-2 Δ18

    Plasmid

    #164436

    Purpose

    Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    SARS-CoV-2 Spike (S )
    

    Expression

    Mammalian

    Available Since

    Feb. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE-APPSw;T719N

    Plasmid

    #177094

    Purpose

    For lentiviral transduction of APPsw; T719N

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    APPsw;T719N (APP Human)
    

    Use

    Lentiviral

    Mutation

    T719N, K651N and M652L (please see depositor comm…

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX6P1-3xMBT_D355N

    Plasmid

    #46988

    Purpose

    Inducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coli

    Depositor

    Or Gozani

    Article

    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

    Insert

    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    

    Tags

    GST and PreScission cleavage site

    Expression

    Bacterial

    Mutation

    Mutation aspartic acid 355 to asparagine (D355N);…

    Promoter

    tac

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 AIF-mClover

    Plasmid

    #129575

    Purpose

    Expression of mClover fused AIF

    Depositor

    Stephen Tait

    Article

    Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

    Insert

    AIF (AIFM1 Human)
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHDM-SARS-CoV-2 Δ18

    Plasmid

    #164434

    Purpose

    Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    SARS-CoV-2 Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGAPTrap-mtagBFP2-IRESMuro

    Plasmid

    #82335

    Purpose

    Vector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    mtagBFP2-IRESMuro
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Tet-O-FUW-STAT4-P2A-mCherry

    Plasmid

    #203853

    Purpose

    Tet-inducible lentiviral plasmid expressing STAT4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    STAT4 (STAT4 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGAPTrap-mCherry-IRESMygro

    Plasmid

    #82505

    Purpose

    Vector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    mCherry-IRESMygro
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-syn-FLEX-synaptoPAC-minWPRE

    Plasmid

    #153100

    Purpose

    Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

    Depositor

    Dietmar Schmitz

    Article

    Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

    Inserts

    bPAC(WT)
    Synaptophysin (Syp Rat)
    

    Use

    AAV

    Tags

    His tag, mScarlet, and myc tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTBL210

    Plasmid

    #126451

    Purpose

    Expresses Cas9-XTEN-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-XTEN-TdT (DNTT Streptococcus pyogenes, Human)
    

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge