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p1195-pssAAV.U1a.hNme2Cas9

Plasmid

#129534

Purpose

AAV vector expressing Nme2Cas9

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

human codon-optimized Nme2Cas9

Use

AAV

Tags

2xNLS and NLS-3xHA-NLS

Promoter

U1a

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMC0x_gRNA_Pos5_Ptet

Plasmid

#179336

Purpose

gRNA Position Vector 5, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pX335-NEAT1_IS_v1

Plasmid

#97085

Purpose

Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1_IS_v1

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCRL1

Plasmid

#153997

Purpose

sgRNA in vitro transcription plasmid.

Depositor

Shengdar Tsai

Article

Lazzarotto et al Nat Protoc. 2018 Nov;13(11):2615-2642. d

Type

Empty backbone

Use

CRISPR; Sgrna under a t7 promoter for in vitro tr…

Promoter

T7

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pBS500 EF1alpha-GFPcre

Plasmid

#11920

Depositor

Brian Sauer

Article

Gagneten et al Nucleic Acids Res. 1997 Aug 15. 25(16):3

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pFlox c-WAPL_EYFP_FKBPF36V_Neo

Plasmid

#163873

Purpose

Acute Depletion of WAPL-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Wapl (Wapl Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pspCas9-3'UTR-ST2-com-vector

Plasmid

#136269

Purpose

Expresses SpCas9 and sgRNA Scaffold

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Insert

SpCas9 and sgRNA scaffold

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX335-NEAT1_IS_v2

Plasmid

#97086

Purpose

Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1_IS_v2

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A3003

Plasmid

#91043

Purpose

Module A, Promoter: CmYLCV, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

CmYLCV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_222

Plasmid

#91116

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFlox c-RAD21_FKBPF36V_EYFP_Bsd

Plasmid

#163871

Purpose

Acute Depletion of RD21-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Rad21 (Rad21 Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRN3P_TIR1

Plasmid

#119766

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

TIR1

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZ143-pcDNA3-BvCas12b

Plasmid

#122445

Purpose

Expresses BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BvCas12b-NLS-3xHA

Tags

3XHA

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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LT2_14_GlyOx

Plasmid

#177711

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
pTRX2_5xUAS-ymYPET-tCYC1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Aurli_150841_GZG

Plasmid

#162563

Purpose

Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

Depositor

Jackie Collier

Article

Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

Insert

shble

Use

Unspecified

Promoter

GAPDH

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCD-Ugi22

Plasmid

#207004

Purpose

pGGC000 Greengate entry vector containing Utricularia gibba insulator 22 (Ugi22) sequence (ID2547)

Depositor

Wayne Parrott

Article

Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.

Insert

Utricularia gibba insulator 22

Use

Golden gate compatible cloning vector

Available Since

Nov. 8, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCD-Ugi21

Plasmid

#207005

Purpose

pGGC000 Greengate entry vector containing Utricularia gibba insulator 21 (Ugi21) sequence (ID9063)

Depositor

Wayne Parrott

Article

Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.

Insert

Utricularia gibba insulator 21

Use

Golden gate compatible cloning vector

Available Since

Nov. 8, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMB4

Plasmid

#122246

Purpose

Lentivirus delivery for stable expression of As crRNA, has AsDR; Cloning vector for expression of AsCpf1 crRNA. It contains BsmBI site for cloning.

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTE1062

Plasmid

#172356

Purpose

Detection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.

Depositor

Eriko Takano

Article

Biarnes-Carrera et al Methods Mol Biol. 2018;1673:117-128. doi

Inserts

scbR (SCO6265 )
kasOp

Use

Synthetic Biology; Integrative into streptomyces …

Expression

Bacterial

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-hCasD10A-pA

Plasmid

#51638

Purpose

Expresses Cas9-nickase in mammalian cells and zygotes.

Depositor

Wataru Fujii

Article

Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

Insert

Codon-optimized Cas9 D10A

Use

CRISPR

Tags

FLAG, NLS, and polyA

Expression

Mammalian

Promoter

CAG promoter

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only