We narrowed to 10,382 results for: plasmids 101

1. mOrange2-Keratin-17

   Plasmid

   #57965

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. CDK4-luciferase active (880)

   Plasmid

   #86657

   Purpose

   Luciferase reporter containing CDK4 promoter

   Depositor

   Jaime Modiano

   Article

   Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

   Insert

   CDK4 promoter (CDK4 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   867 base pairs including up to coding nucleotide …

   Promoter

   CDK5

   Available Since

   June 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. CDk4-luciferase fragment A

   Plasmid

   #86658

   Purpose

   Luciferase reporter containing CDK4 promoter

   Depositor

   Jaime Modiano

   Article

   Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

   Insert

   CDK4 promoter (CDK4 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   generated by cleavage at the NcoI site from 880 (…

   Promoter

   CDK6

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. hsa-cdk2 3'UTR sicheck2

   Plasmid

   #121125

   Purpose

   luciferase assay

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   hsa-cdk2 3'UTR (CDK2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCRII apoA-Ib

   Plasmid

   #121896

   Purpose

   Plasmid for making zebrafish apoA-Ib in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   ApoA1b (apoa1b Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CDk4-luciferase fragment B

   Plasmid

   #86659

   Purpose

   Luciferase reporter containing CDK4 promoter

   Depositor

   Jaime Modiano

   Article

   Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

   Insert

   CDK4 promoter (CDK4 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   generated by cleavage at the KpnI site from 880 (…

   Promoter

   CDK7

   Available Since

   June 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCerulean3-Keratin-17

   Plasmid

   #55424

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

8. TUBG1_pLX307

   Plasmid

   #98376

   Purpose

   Lentiviral expression of TUBG1

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   TUBG1 (TUBG1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9/pTREX-n

   Plasmid

   #68708

   Purpose

   Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Insert

   Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
   

   Use

   CRISPR

   Tags

   Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

   Expression

   Mutation

   C4239T mutation that eliminates a BamHI restricti…

   Promoter

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. tetO-ESRRA

    Plasmid

    #170683

    Purpose

    doxycycline-inducible overexpression of ESRRA

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    ESRRA (ESRRA Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LIMD1_pLX307

    Plasmid

    #98349

    Purpose

    Lentiviral expression of LIMD1

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    LIMD1 (LIMD1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-shCasp2-IRES-GFP

    Plasmid

    #52061

    Purpose

    stable knockdown of Caspase-2 in mammalian cells

    Depositor

    Tyler Jacks, Trudy Oliver

    Article

    Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

    Insert

    Caspase-2 shRNA (CASP2 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pmirGlo-RBM8A-3'UTR 240-546

    Plasmid

    #158108

    Purpose

    reporter 3'UTR hRBM8A (short)

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    3'UTR of hRBM8A (RBM8A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hIBA1a-GFP-miR124T

    Plasmid

    #214147

    Purpose

    AAV vector to restrict GFP expression in microglia.

    Depositor

    Chun-Li Zhang

    Article

    Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

    Has Service

    AAV5

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Cre

    Plasmid

    #13775

    Purpose

    Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-ERT2CreERT2

    Plasmid

    #13777

    Purpose

    Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    ERT2-Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. AP1

    Plasmid

    #179678

    Purpose

    Accessory plasmids

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [SD8] gIII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSF1389

    Plasmid

    #104962

    Purpose

    Bacterial expression plasmid of bdSENP1 protease

    Depositor

    Dirk Görlich

    Article

    Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

    Insert

    SENP1
    

    Use

    Tags

    His14 tag and Tev protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV VIVIT-GFP

    Plasmid

    #188707

    Purpose

    Lentivirus transfer plasmid encoding VIVIT-GFP

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    VIVIT-GFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-CRISPR

    Plasmid

    #160047

    Purpose

    For cloning sgRNA to knockout a specific gene in piggybac plasmid

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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