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Flag-HA-PSMD14

Plasmid

#22557

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

PSMD14 (PSMD14 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pBPGAL4.2Uw-2

Plasmid

#26227

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV SOCS-3

Plasmid

#11486

Depositor

Ronald Kahn

Article

Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

Insert

SOCS-3 (Socs3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S EN2L

Plasmid

#20922

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Lin28 (POU5F1, SOX2, NANOG, LIN28A Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pBABE-FLAG-KD LKB1

Plasmid

#8593

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K78I: lysine 78 mutated to isoleucine; kinase dead

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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pLVPT-tTR-KRAB

Plasmid

#11642

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.1

Plasmid

#32445

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Expression

Mammalian

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLPC myc hPOT1

Plasmid

#12387

Depositor

Titia de Lange

Article

Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Insert

Protection of Telomeres 1 (POT1 Human)

Use

Retroviral

Tags

Myc

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP16

Plasmid

#141269

Purpose

Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pPGK-Cre-bpA

Plasmid

#11543

Purpose

Expresses Cre recombinase from mouse PGK promoter in mammalian cells.

Depositor

Klaus Rajewsky

Insert

Phosphoglycerate kinase 1 promoter

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

Plasmid

#141387

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf6 (ORF6 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro

Plasmid

#141392

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf9b

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro

Plasmid

#141369

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp4 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDIRE

Plasmid

#26745

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Insert

iCre expression cassette

Use

Cre/Lox; Flp/frt

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG2LMKOSimO

Plasmid

#20866

Depositor

Keisuke Kaji

Article

Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

Insert

c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2-ires-mOrange (Myc Mouse)

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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PGKneotpAlox2

Plasmid

#13444

Depositor

Philippe Soriano

Article

Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Insert

PGKneotpAlox2

Use

Cre/Lox

Expression

Mammalian

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pSico PGK puro

Plasmid

#11586

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

Plasmid

#20943

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

H134R

Promoter

CaMKIIa

Available Since

July 9, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-DRD2

Plasmid

#24099

Depositor

Jean-Michel Arrang

Article

Jeanneteau et al Mol Biol Cell. 2004 Feb . 15(2):696-705.

Insert

DRD2 (DRD2 Human)

Tags

alpha7 nicotinic signal peptide and eGFP

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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IL-2 promoter luciferase

Plasmid

#12194

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-2 promoter (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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