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pHCMV_MVEV_PrME

Plasmid

#207295

Purpose

Mammalian expression of Murray valley encephalitis virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

MVEV_PrME

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_CHHF_GPC

Plasmid

#207260

Purpose

Mammalian expression of Chapare hemorrhagic fever virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

CHHF_GPC (CVsS_gp1 Chapare hemorrhagic fever virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_E2-Crimson

Plasmid

#62410

Purpose

MXS_chaining vector with E2-Crimson

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

E2-Crimson

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR1A-NTAP-A (w322-1)

Plasmid

#17402

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

CBP and SBP

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR1A-FKBP12DD C-term (w350-2)

Plasmid

#17413

Purpose

Gateway entry vector for Degron, C-term

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

FKBP12DD (L106P)

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBW842_pCAG-pExpr-FP-DECODER

Plasmid

#87551

Purpose

Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mtagBFP, EGFP, iRFP720, mRuby2

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pPD128 AND Gate, 3 repeats

Plasmid

#138937

Purpose

EYFP reporter vector for the Logic 37 AND 158 NOT 43 3 repeats promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 158 NOT 43 3 repeats

Use

Synthetic Biology

Expression

Mammalian

Promoter

Logic 37 AND 158 NOT 43 3 repeats

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-W340A.pcDNA3-k

Plasmid

#127404

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon and W34…

Promoter

CMV

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTYF-mCMV/SYN-EGFP

Plasmid

#19975

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

mCMV/SYN-EGFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pIM573

Plasmid

#74637

Purpose

alias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

ymTagBFP

Expression

Yeast

Promoter

none

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Golgi-Ras-LOCKR-S

Plasmid

#216499

Purpose

FRET-based reporter for Ras activity, Golgi localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Golgi-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 ER-Ras-LOCKR-S

Plasmid

#216500

Purpose

FRET-based reporter for Ras activity, ER localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

ER-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Mito-Ras-LOCKR-S

Plasmid

#216501

Purpose

FRET-based reporter for Ras activity, mitochondria localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Mito-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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34a_PU1 in pmiRGlo

Plasmid

#78131

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

PU1

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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MTK3_036

Plasmid

#123750

Purpose

Encodes mScarlet as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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myr-palm_mCherry2-mEGFP

Plasmid

#127816

Purpose

Hetero-dimer mCherry2-mEGFP expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

myristoylated-palmitoylated-mCherry2-mEGFP

Expression

Mammalian

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-APC11

Plasmid

#19900

Depositor

Yue Xiong

Article

Zacharek et al Cancer Res. 2005 Dec 15. 65(24):11354-60

Insert

APC11 (Anaphase promoting complex subunit 11) (ANAPC11 Human)

Tags

HA

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pGL2B -938/+64

Plasmid

#24943

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pCGN R-Ras wt

Plasmid

#14727

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

Insert

R-ras (RRAS Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
p-dCas9-p300-Hygro

Plasmid

#104411

Purpose

transient expression of dCas9-p300 fusion protein

Depositor

Stephan Beck

Insert

p300 (EP300 Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pECFP(C1)-PP1gamma

Plasmid

#44232

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP-1G (PPP1CC Human)

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pPAGFP-TPX2

Plasmid

#31226

Depositor

Patricia Wadsworth

Article

Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

Insert

TPX2 (TPX2 Human)

Tags

PAGFP

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
CLPIT Cry2PHR-mCh-LRP6c

Plasmid

#42961

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-LRP6c

Use

Retroviral

Expression

Mammalian

Mutation

LPR6 cytoplasmic domain only

Promoter

Tet-Off promoter

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-GFP26

Plasmid

#106423

Purpose

Y-RAN-GFP26 (RANbody GFP26::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-GFP26

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalB S28N

Plasmid

#19722

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog B (RALB Human)

Use

Retroviral

Expression

Mammalian

Mutation

S28N for dominant negative function.

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pQCXP CMV/TO LUC (w432-1)

Plasmid

#17476

Purpose

Retroviral Luciferase expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Luciferase and Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBS-IT6

Plasmid

#66870

Purpose

Inntag 6: a small self-structured epitope aimed at innocuous protein tagging

Depositor

Marti Aldea

Article

Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

Inntag 6

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalB Q72L

Plasmid

#19721

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog B (RALB Human)

Use

Retroviral

Expression

Mammalian

Mutation

Q72L constitutively active, GTPase Deficient

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
p38delta

Plasmid

#29575

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

p38delta (Mapk13 Mouse)

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Starting Met replaced with Ala

Available Since

July 13, 2011

Availability

Academic Institutions and Nonprofits only
pT7-M1-S1-S2-S4T1L

Plasmid

#33306

Depositor

Terence Dermody

Article

Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

Inserts

S2 (MoV1sS2gp1 Orthoreovirus T1L)
S1 (MoV1sS1gp2, MoV1sS1gp1 Orthoreovirus T1L)
S4 (MoV1sS4gp1 Orthoreovirus T1L)
M1 (M1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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