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LT1_30_backbone_X2_integration

Plasmid

#194403

Purpose

Multi Backbone with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_31_backbone_HO_integration

Plasmid

#194404

Purpose

Multi Backbone with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPD95.77-wVenus

Plasmid

#37465

Depositor

Hitoshi Sawa

Article

Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

Type

Empty backbone

Expression

Worm

Promoter

none

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pET29b-NpR5599

Plasmid

#45740

Depositor

Emily Balskus, Christopher T Walsh

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

NpR5599 (Npun_R5599 Nostoc punctiforme)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
EC70128

Plasmid

#209489

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC70214

Plasmid

#209490

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70125

Plasmid

#209486

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70127

Plasmid

#209488

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70126

Plasmid

#209487

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70212

Plasmid

#209484

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70213

Plasmid

#209485

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMK-nptII

Plasmid

#123560

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

nptII

Use

Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pYTK096

Plasmid

#65203

Purpose

Pre-Assembled Ura3 integration vector to be used in the Dueber YTK system.

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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lvl0 HSPt

Plasmid

#153378

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

HSP terminator

Expression

Plant

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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oxVenus

Plasmid

#68067

Purpose

C-terminal fusion to oxVenus

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Tags

oxVenus

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
EBFP2-CathespinB-6

Plasmid

#55236

Purpose

Localization: Lysosomes, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Article

Sundaresan et al J Clin Oncol. 1987 Nov;5(11):1739-45.

Insert

CathespinB

Tags

EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSD02

Plasmid

#16009

Depositor

Fred Cross

Article

Di Talia et al Nature. 2007 Aug 23. 448(7156):947-51.

Insert

ACT1pr-DsRed.T1.N1

Expression

Yeast

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pSEVAD1K1A

Plasmid

#58299

Purpose

GeneGuard vector cassette: dapA, R6K COR, Kid toxin; with ampR as cargo.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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lvl0 ACT7p

Plasmid

#153371

Purpose

Plant promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

ACT7 promoter

Expression

Plant

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBlueScript NCX1.1

Plasmid

#66799

Depositor

Michela Ottolia, Kenneth Philipson

Article

Nicoll et al Science. 1990 Oct 26;250(4980):562-5.

Insert

NCX1.1

Use

T3 transcription of gene

Mutation

there are 3 silent mutations within the open read…

Promoter

T3

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-HCN1miR-EGFP-L2

Plasmid

#32585

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Intronic HCN1miRNA and EGFP

Use

Gateway cloning vector

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pG20_FLAG_Hyg

Plasmid

#159713

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Tags

FLAG

Expression

Plant

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Auxiliary A

Plasmid

#153352

Purpose

Provides End-to-End linker 4A for level 2 cloning

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

End-to-End linker 4A

Use

Synthetic Biology

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary 2

Plasmid

#153350

Purpose

Provides Middle-to-End linker 2 for level 2 cloning

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

Middle-to-End linker 2

Use

Synthetic Biology

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 UBQ5t

Plasmid

#153383

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

UBQ5 terminator

Expression

Plant

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-FKBP:mCherry-KRas4Bc30

Plasmid

#86633

Purpose

Expresses mCherry-tagged membrane recruiter construct

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

FKBP:mCherry-KRas4Bc30

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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CMVTO-g0

Plasmid

#179649

Purpose

Non-coding DNA plasmid control

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

g0

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pG20_Venus_Blp

Plasmid

#159704

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Tags

mVenus

Expression

Plant

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMK-mEmerald

Plasmid

#123556

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

mEmerald

Use

Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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28 pCS plcg 1

Plasmid

#13059

Depositor

Nathan Lawson

Article

Lawson et al Genes Dev. 2003 Jun 1. 17(11):1346-51.

Insert

plcg1 (plcg1 Zebrafish)

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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