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pBABEpuro-ARAF_S214C

Plasmid

#51077

Purpose

mammalian expression of ARAF S214C

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Mutation

S214C

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Del-7

Plasmid

#14968

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-26/+334) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-26/+334 (relative to the P2 transcription initia…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 merlin 308-590

Plasmid

#11627

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Carboxy-terminal half of merlin isoform 2.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pBV-Luc/Frag-F

Plasmid

#14053

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/-3) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-109/-3 (relative to the P2 transcription initiat…

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pQCXIpuro-H3.3K4M-FLAG/HA

Plasmid

#128742

Purpose

Expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Mutation

K4M

Promoter

CMV

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTox-EGFPsite1_NGA-PAM (BPK754)

Plasmid

#181747

Purpose

p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)

Use

Selection plasmid

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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AcrIIC1X

Plasmid

#128112

Purpose

AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrX (AcrIIC1 N3F/D15Q/A48I)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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p6900 PHAGE-P CMVT N-HA-hE6AP III C840A

Plasmid

#37606

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP III C840A (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C840A

Promoter

CMV tet regulatable

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pVLAD6-mmPtch1-B-SBP

Plasmid

#120886

Purpose

BacMam vector for Ptch1-B expression

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

SBP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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(81) pcDNA3.1-BC2-Nup62-Flag-EPEA

Plasmid

#168094

Purpose

Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

Nup62-Ubc-Flag-EPEA

Tags

BC2-Tag, EPEA-tag, and Flag-tag

Expression

Mammalian

Mutation

P229A, S283T on Nup62 native sequence

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET30a-ASXL1-HARE-HTH domain

Plasmid

#107164

Purpose

Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coli

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

ASXL1 (Asxl1 Mouse)

Tags

Flag

Expression

Bacterial

Mutation

Contains amino acids 2-83

Promoter

T7

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-His6-Mps1-KD

Plasmid

#114064

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

Insert

Mps1-KD (TTK Human)

Tags

His6

Expression

Insect

Mutation

Mps1 KD (D664A)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-NNQ-HA-GFP

Plasmid

#121164

Purpose

Expresses mmPtch1-B NNQ variant

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, N…

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFP

Plasmid

#120909

Purpose

Expresses Ptch1-B (I766FV111FL114F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_HGS_VHS

Plasmid

#109890

Purpose

Protein expression and purification of HGS_VHS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

HGS_VHS (HGS Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 merlin 308-579

Plasmid

#11626

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Carboxy-terminal common domain of merlin.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
GFP-Clavesin2 (pEGFP-C1-Sec14b)

Plasmid

#47420

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin2 (CLVS2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-h-mmPtch1-B-L235C-HA

Plasmid

#120906

Purpose

Expresses mmPtch1-B (L235C)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, L…

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCascade-IL1RNcrRNA

Plasmid

#126493

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting IL1RN

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Lmo IL1RN cr03

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCascade-HBE1crRNA

Plasmid

#126494

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting HBE1

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Lmo HBE1 cr06

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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