We narrowed to 53,406 results for: plasmid

1. p-dCas9-G9a-Hygro

   Plasmid

   #104408

   Purpose

   transient expression of dCas9-G9a fusion protein

   Depositor

   Stephan Beck

   Insert

   G9a (EHMT2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPS-hSox2-gfp

   Plasmid

   #49390

   Purpose

   mammalian expression of human Sox2 fused to EGFP

   Depositor

   Randall McKinnon

   Insert

   Sox2 (SOX2 Human)
   

   Use

   Cre/Lox; Acceptor vector

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   TGA (stop) to CCA (Pro) at nt 1027 with respect t…

   Promoter

   CMV

   Available since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4134547

   Plasmid

   #152583

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;HA

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   July 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pBBR5pemIKpKan

   Plasmid

   #129271

   Purpose

   Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and constitutive promoter

   Depositor

   Lindsey Burbank

   Article

   Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-GluA1-scFv-Halo

   Plasmid

   #230055

   Purpose

   AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA1 single chain variable fragment (scFv)
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBW-m4133502

   Plasmid

   #153565

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;HA

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAH121

   Plasmid

   #164901

   Purpose

   The pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

   Insert

   phi21 Integrase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBW-m4133717

   Plasmid

   #152919

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;HA

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   July 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. huntingtin_FL_NbioCflag

   Plasmid

   #194767

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   Avi-tag (biotin acceptor peptide) and Flag-tag

   Expression

   Mammalian

   Mutation

   Q23 (polyQ repeat)

   Promoter

   CMV and P10

   Available since

   July 27, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGBW-m4134336

    Plasmid

    #152014

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBBR5pemIKpBAD

    Plasmid

    #129273

    Purpose

    Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and arabinose-inducible promoter

    Depositor

    Lindsey Burbank

    Article

    Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHG6

    Plasmid

    #188254

    Purpose

    Integrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selection

    Depositor

    Maggie Smith, Hong Gao

    Article

    Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    act1p/actII-orf4

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. EPB41L3A-c000

    Plasmid

    #175458

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-D1087.

    Depositor

    Opher Gileadi

    Insert

    EPB41L3 (EPB41L3 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TH mAb_BCN53.1.1D10_HC_Mouse IgG2a

    Plasmid

    #182896

    Purpose

    Mammalian expression of TH mouse mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-TH (human) heavy chain (TH Mouse)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4133361

    Plasmid

    #154789

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pHG7

    Plasmid

    #188255

    Purpose

    Integrating plasmid contains the C31 int/attP locus and the hygromycin-resistance gene for selection

    Depositor

    Maggie Smith, Hong Gao

    Article

    Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    act1p/actII-orf4

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBACgus4x/cdc37/LRRK2 1867-2186

    Plasmid

    #40364

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    HisTev

    Expression

    Mutation

    WT aa1867-2186

    Promoter

    polH

    Available since

    Oct. 2, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pJPC13

    Plasmid

    #80773

    Purpose

    Cloning vector compatible with ColE1 plasmids with medium copy number

    Depositor

    Gregory Phillips

    Article

    Peterson et al Plasmid. 2008 May;59(3):193-201. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIB190

    Plasmid

    #90197

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBW-m4133197

    Plasmid

    #152383

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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