We narrowed to 6,546 results for: proc

1. MSCV-TAP(FLAG-HA) CYLD

   Plasmid

   #60031

   Purpose

   CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Retroviral

   Tags

   FLAG, HA, and IRES Puro

   Expression

   Mammalian

   Mutation

   Promoter

   LTR-driven expression

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pET28_6xHis-PARP1-W318R

   Plasmid

   #174793

   Purpose

   Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-W318R (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   W318, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. HOXB4 (human) HA-TAT-tag pET

   Plasmid

   #8526

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB4 (HOXB4 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial

   Mutation

   replace T7 and HIS tags of pET with an HA tag;

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

4. J23114-mRFP1-331Bb

   Plasmid

   #78279

   Purpose

   mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Insert

   J23114-mRFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX4T2 gp78 G2BR mt / JM26

   Plasmid

   #13308

   Depositor

   Allan Weissman

   Article

   Chen et al Proc Natl Acad Sci U S A. 2006 Jan 10. 1

   Insert

   gp78 (AMFR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   cDNA corresponds to aa309-643 derived from human …

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pCS2-hFAM 3B-His

   Plasmid

   #172192

   Purpose

   Coding sequence of human FAM3B pCS2 C terminal 6xHis tag used for protein purification from conditioned medium

   Depositor

   Edward De Robertis

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

   Insert

   hFAM 3B
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p6589 MSCV-IP N-HAonly CHEK2

   Plasmid

   #34903

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   CHEK2 (CHEK2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pDN-T1TCbt

   Plasmid

   #44514

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
   tetR::ymcherry
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Three tandem tet operators (3XtetO2) downstream o…

   Promoter

   PGal1-T123 promoter

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNXgate33-3HA

   Plasmid

   #105075

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

   Depositor

   Wolf Frommer

   Article

   Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

   Type

   Empty backbone

   Use

   Tags

   3xHA and NubG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRShygro shID3#1

    Plasmid

    #19164

    Depositor

    Joan Massague

    Article

    Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

    Insert

    small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3/flag-pBirA(1-G78)-FRB

    Plasmid

    #154447

    Purpose

    expresses pBirA(1-G78)-FRB/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    flag-pBirA(1-G78)-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBabe 3xFLAG mouse Bmf

    Plasmid

    #17243

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bmf (Bmf Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

13. nuc-yHS1-M7A,H102A

    Plasmid

    #159168

    Purpose

    Nuclear labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    nuc-yHS1-M7A,H102A
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDN-G1TCbt

    Plasmid

    #44515

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::ymcherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mKikGR-Tubulin-6

    Plasmid

    #57330

    Purpose

    Localization: Microtubules, Excitation: 507 / 583, Emission: 517 / 593

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    mKikGR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pHR-ZIP(FF)

    Plasmid

    #27135

    Depositor

    Ron Vale

    Article

    Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

    Insert

    CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    eGFP and mCherry

    Expression

    Mammalian

    Mutation

    Tyrosines in ITAMS 1-3 mutated to phenylalanines …

    Promoter

    Available since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSN672

    Plasmid

    #177362

    Purpose

    Expresses human KIF1A(1-393) fused with leucine zipper and His tag

    Depositor

    Shinsuke Niwa

    Article

    Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

    Insert

    KIF1A (KIF1A Human)
    

    Use

    Tags

    His tag and Leucine zipper

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA FLAG mTOR ST

    Plasmid

    #22999

    Depositor

    Jing Huang

    Article

    Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 7. ()

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2035T

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pET22b-ecDHFR-N23PP/L28F/G51PEKN

    Plasmid

    #118585

    Purpose

    Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, L28 mutated to F28, G51 mutat…

    Promoter

    T7

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAD108_Arg273His_pCMV6Entry-hIRG1

    Plasmid

    #124878

    Purpose

    Expresses hCAD Arg273His mutant in mammalian cells

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    myc and FLAG tags

    Expression

    Mammalian

    Mutation

    mutated Arg273 to His

    Promoter

    CMV

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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